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The pike-perch (Sander lucioperca L.) at the age of 18 days were fed for 21 days using three different diets: Agio Norse (An), casein-gelatin (Cas), cod meal with gelatin (Mac) and nauplius Artemia salina (Art - control diet). On the last day of the experiment, fish fed Art and An diets had the statistically significant highest body mass, length, and survival. On the last day, the highest aminopeptidase activity in the anterior intestine and posterior intestine was registered in fish fed nauplius Artemia salina. The lowest activity of this enzyme in the anterior intestine was to be found in fish fed with Cas diet, while there was no difference between among groups in the posterior intestine. The non-specific esterase activity was registered in the stomach, liver, anterior intestine as well as in the posterior intestine. The lowest activity of this enzyme in the stomach was observed in the pike-perch fed with the Cas diet. In the anterior intestine, the highest activity was registered in fish fed with Art, and the lowest - on the Cas and Mac diet. The results of the current research prove that feeding the pike-perch An diet has a positive effect of the survival of the pike-perch, their growth rate and the activity of the enzymes. By contrast, feeding pike-perch Cas and Mac diets did not satisfy nutritional needs of fish, resulting in their low survival, growth rate and low activity of the enzymes examined.
The study focuses on the activity of alkaline phosphatase, acidic phosphatase and non-specific esterase in the epithelial cells of the oviducts of puerperal ewes with a single lamb (n = 24) and two lambs (n = 24). The experimental animals had the ampullar sections of the oviducts removed on days 7, 17, 25 and 35 postpartum. The results indicated an upward trend in the activity of the investigated enzymes in both groups of animals from day 7 to 35 of the puerperal period. However, the increase in enzyme activity in the ewes with twins was slower in comparison with the ewes with a singleton, which was significantly manifested by lower alkaline phosphatase activity (5.8 ± 0.4 vs 4.3 ± 0.4; P < 0.01), acidic phosphatase activity (11.5 ± 0.3 vs 7.2 ± 0.4; P < 0.05) and non- -specific esterase activity (11.3 ± 0.4 vs 8.7 ± 0.4; P < 0.05) on day 25 postpartum. These differences between the groups persisted until the completion of the experiment with alkaline phosphatase activity (7.1 ± 0.7 vs 5.7 ± 0.3; P < 0.05), acidic phosphatase activity (14.4 ± 0.4 vs 8.6 ± 0.3; P < 0.001) and non-specific esterase activity (11.6 ± 0.5 vs 9.5 ± 0.4; P < 0.01) on day 35 postpartum. The results indicate that the retardation of functional changes in the ewes with twins reveals the prolongation of time essential for the completion of regenerative processes in the epithelium of the oviducts. The discussion deals with the possible utilization of these facts in relation to optimizing out of season breeding in sheep.
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