Wyniki wyszukiwania

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High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara

100%

Thirugnanasampandan R., Mahendran G., Narmatha Bai V.

Acta Physiologiae Plantarum

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2010

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tom 32

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nr 2

A protocol for in vitro propagation of Isodon wightii (Bentham) H. Hara from nodal segments was developed. Multiple shoots were successfully established on half strength MS medium supplemented with 4.4 µM BA. Enhancement of shoot multiplication and elongation was achieved on half strength MS medium supplemented with 4.4 µM BA and 1.4 µM GA₃. The regenerated shoots were rooted successfully on half strength MS medium supplemented with 4.9 µM IBA. Acclimatization of in vitro rooted shoots was successful. The in vitro regenerated plants grew well in the greenhouse without any phenotypic changes.

2

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Fast direct regeneration of plants from nodal explants of Stevia rebaudiana Bert.

72%

Dolinski R., Kowalczyk K.

Acta Scientiarum Polonorum. Hortorum Cultus

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2019

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tom 18

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nr 5

In the preceding research, stevia has been typically cloned in vitro using two media, on which the shoots were formed (3–6 weeks), and on the other they were rooted (3–5 weeks). This study aimed at finding the possibility for rapid stevia propagation from large nodal explants using the MS basal medium [Murashige and Skoog 1962], with low auxin concentrations (0.5, 1 and 2 mg.dm–3). The plants were obtained as soon as after three weeks. The best results were obtained from media with various concentrations of the indole-3-acetic acid (IAA) and the highest concentration of phenylacetic acid (PAA). Plants were formed by 83.9–86.0% of explants, they had high weight (234–253 mg), two shoots measuring 2.07–2.37 cm and 5.8–8.3 roots measuring 1.00–1.24 cm. Mean plant weight was the lowest on media with indole-3-butyric acid (IBA) (185–192 mg). Both explant buds formed single shoots, but their development was typically uneven. The differences in the length and weight of shoots were the lowest on media with IAA and at lower PAA concentrations. Plants from the media with IAA and the control medium were distinguished by higher number of nodes. The percentage share of shoots in the total plant weight was the highest on media with PAA (62.1–62.7%), and the lowest at higher concentrations of α-naphthaleneacetic acid (NAA) (47.9 and 48.9%). Parts of explants immersed in media developed callus, and the highest amounts of this tissue were found in the media with NAA. 92.3% of plants survived the acclimatization. The applied procedure may be used for rapid in vitro cloning of selected stevia genotypes. The use of one medium enables reduction of seedling production costs. Moreover, cyclical cloning and extending the production scale is possible.

3

Micropropagation of Pluchea lanceolata (Oliver and Hiern.) using nodal explant

72%

Kher M. M., Joshi D., Nekkala S., Nataraj M., Raykundaliya D. P.

Journal of Horticultural Research

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2014

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tom 22

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nr 1

4

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

72%

Li J., Kang Y., Qiang S., Peng G.

Acta Societatis Botanicorum Poloniae

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2012

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tom 81

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nr 1

Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog's medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation, rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.

5

Lofty frequency and reproducible plant regeneration from mature nodal explants of ‘‘Egusi’’melon (Citrullus colocynthis L.)

72%

Thangavel P., Natarajan S., Shanmugam V., Sulaiman S. M., Ramasamy R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

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nr 3

6

Shoot multiplication and plant regeneration of guava [Psidium guajava L.] from nodal explants on in vitro raised plantlets

58%

Rai M. K., Jaiswal V. S., Jaiswal U.

Journal of Fruit and Ornamental Plant Research

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2009

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tom 17

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nr 1

29-38

A micropropagation method is described for guava (Psidium guajava L.) using nodal expiants from somatic embryo-derived young and aseptic plantlets. Multiple shoots were induced from axillary buds on MS medium containing different concentrations of N6-benzylaminopurine (BAP), either alone or in combination with kinetin (Kn), indole-3-acetic acid (1AA) or a-naphthalene acetic acid (NAA). Medium containing 1 mg l-1 BAP was the most effective for shoot multiplication. In vitro regenerated shoots developed roots either on MS medium alone or on MS medium supplemented with indole-3-butyric acid (1BA). The rooted plantlets were successfully acclimatized.

7

Micropropagation of Merremia quinquefolia (l.) Hallier F. from nodal explants

58%

Kher M. M., Nataraj M., Parmar H. D., Buchad H.

Journal of Horticultural Research

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2015

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tom 23

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nr 1

Ograniczanie wyników

High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara

Fast direct regeneration of plants from nodal explants of Stevia rebaudiana Bert.

Micropropagation of Pluchea lanceolata (Oliver and Hiern.) using nodal explant

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

Lofty frequency and reproducible plant regeneration from mature nodal explants of ‘‘Egusi’’melon (Citrullus colocynthis L.)

Shoot multiplication and plant regeneration of guava [Psidium guajava L.] from nodal explants on in vitro raised plantlets

Micropropagation of Merremia quinquefolia (l.) Hallier F. from nodal explants