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The objective of the study was to determine the direction, intensity and duration of changes in abundance and activity of certain microbial groups active in nitrogen transformations in soil subjected to a 4-year fertilization treatment with municipal and industrial sewage sludge. The study was conducted on podzolic soil, whose accumulation horizon had been fertilized in 1998 with fermented sewage sludge at doses of 30 Mg⋅ha-1(1%), 75 Mg⋅ha-1(2.5%), 150 Mg⋅ha-1(5%), 300 Mg⋅ha-1(10%) and 600 Mg⋅ha-1(20%) and planted with willow (Salix viminalis L.). Four years after the application of sludge, microbiological and biochemical analyses were made in two soil horizons (0-20 cm and 20-40 cm). It was found that in the soil from the 0-20 cm depth significant stimulation of the growth of proteolytic fungi and bacteria continued, but only under the effect of the highest dose of sludge. Moreover, there was a notable stimulation of protease activity and nitrification process alongside a slight inhibition of ammonification. In the soil from the 20-40 cm layer stimulation of the growth of protein-decomposing fungi was observed as well as that of proteolytic and nitrification activity of soil, while ammonification was inhibited. However, the effect of sludge was generally less pronounced in the deeper soil layer than in the surface soil horizon.
Intensity of ammonification, nitrification (NH4-N oxidation to N02-N) and denitrification (reduction of NO3-N to NO2-N) was studied in soils experimentally contaminated with oil and control soils. Studies were carried out on 144 experimental plots of total area 1 ha. Half of the plots were cultivated in the usual way, the other half - intensively. Each of the 2 combinations comprised plots fertilized only with NPK, NPK and manure, and with NPK, manure and additionally inoculated with bacteria - decomposing petroleum hydrocarbons. Rye, barley, a mixture of legumes and cereals, and potatoes were cultivated in all plot combinations. Intensity of ammonification, nitrification and denitrification was determined in soil samples collected in spring, 24 months after soil contamination with petroleum. Ammonification was most intensive in soil samples collected from plots polluted with crude oil, fertilized with NPK and manure, inoculated with bacteria strains decomposing petroleum, in which rye was cultivated. NH4-N oxidation to N02-N was most intensive in soil samples collected from plots which had not been contaminated with oil, fertilized with NPK and manure, in which potatoes were cultivated. The highest reduction of N03-N to NO2-N was observed in soil polluted with oil, fertilized with NPK, in which potatoes were planted and cultivated in the usual way.
The response of soil microflora to increasing concentration of three pesticide preparations containing diazinon (insecticide), linuron (herbicide) and mancozeb + dimethomorph (fungicide) as an active substances, was determined under laboratory conditions. All pesticides were applied at three different dosages, i.e. 7, 35 and 700 mg kg⁻¹ soil for the insecticide, 4, 20 i 400 mg kg⁻¹ soil for the herbicide and 15, 75 and 1500 mg kg⁻¹ soil for the fungicide mixture. The lowest dosages corresponded to the maximum predicted environmental concentration (PEC) of pesticides applied in field conditions, while the other dosages were the multiplicities of PEC. After 1, 14 and 28 days of experiment the numbers of heterotrophic bacteria, fungi and bacteria involved in nitrogen transformation (nitrifying, denitrifying and N₂ -fixing bacteria) was estimated. The numbers of colony forming units (CFU) of respective groups of microorganisms were determined on selective media by means of the serial dilution technique and the spread plate method. The plate-count data indicated that pesticide used affected the numbers of tested microbial groups. All chemicals stimulated the numbers of heterotrophic bacteria and fungi at the higher dosages. However, the reverse effect was detected at the beginning of the experiment. In turn, bacteria involved in nitrogen turnover, particularly N₂ - fixing and nitrifying bacteria, were the most sensitive to the tested pesticides and their numbers were significantly reduced on each time-point. This great susceptibility of these physiological groups of microorganisms predispose them to be warning biomarkers for the side-effects of environmental pollution caused by chemicals.
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