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NADH-generating substrates reduce peroxyl radical toxicity in RL-34 cells

100%

Antosiewicz J., Spodnik J. H., Teranishi M., Herman-Antosiewicz A., Kurono C., Soji T., Wozniak M., Borkowska A., Wakabayashi T.

Folia Morphologica

2009

tom 68

nr 4

247-255

There is general agreement that oxidative stress may induce apoptotic and necrotic cell death. Recently it has been shown that NADH can be considered an important antioxidant as it reacts with peroxyl and alkoxyl radicals under in vitro conditions. Therefore, in the present study we hypothesized that an increase in intracellular NADH using specific substrates will protect RL-34 cells against cytotoxicity of 2’-azobis (2-amidinopropane) dihydrochloride (AAPH), which is a peroxyl radical generating compound. Cells treated for 24 hours with 6.0 mM AAPH were severely damaged: mitochondria were vacuolated, and the level of free radicals significantly increased. Both apoptotic and necrotic cells were detected (11.1% and 11.4%, respectively) even after 5 hours of treatment. Pretreatment of the cells with substrates which increase the intracellular level of NADH, such as lactate, beta-hydroxybutyrate, and ethanol, distinctly inhibited AAPH-induced reactive oxygen species (ROS) formation and cell death. On the other hand, acetoacetate (AcA), which decrease the intracellular level of NADH, had opposite effects. Interestingly, NADH-generating substrates augment, while AcA reduced superoxide radical formation induced by AAPH. These results may suggest that although NADH generating substrates may exert some deleterious effects within a cell by inducing reductive stress, they diminish alkoxyl or peroxyl radical cytotoxicity. The protection is associated with a decrease in ROS formation measured by dichlorofluorescein, but with an increase in superoxide radical formation. (Folia Morphol 2009; 68, 4: 247–255)

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Methodology of Petri networks for simultaneous evaluation of the impact of different modifiers on the fluorescence of nucleotides from electron transport chain in isolated mitochondria and on the process of swelling

100%

Danylovych H., Chunikhin A., Danylovych Y., Kosterin S.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2018

tom 99

nr 1

Genes and physical fitness

80%

Gronek P., Holdys J.

Trends in Sport Sciences

2013

tom 20

nr 1

The search for genes with that positively affect physical fitness is a difficult process. Physical fitness is determined by numerous genes, and its genetic determinants are modified by environmental factors. The map of candidate genes that can potentially affect physical fitness becomes larger every year, and currently it contains more than 200 genes associated with such aspects as respiratory and cardiovascular stability; body build and composition – especially muscle mass and strength; carbohydrate and lipid metabolism; response to training; and exercise intolerance. The inclusion of the genetic component in physiological and biochemical studies would permit drawing a representation of predispositions for each athlete interested in practicing high performance sports and would be a valuable coaching aid in the process of training individualization.

Ograniczanie wyników

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Folia Morphologica

1 Trends in Sport Sciences

1 Antosiewicz J.

1 Borkowska A.

1 Chunikhin A.

1 Danylovych H.

1 Danylovych Y.

1 Gronek P.

1 Herman-Antosiewicz A.

1 Holdys J.

1 Kosterin S.

1 Kurono C.

1 Soji T.

1 Spodnik J. H.

1 Teranishi M.

1 Wakabayashi T.

1 Wozniak M.

1 2018

1 2013

1 2009

Wyniki wyszukiwania

NADH-generating substrates reduce peroxyl radical toxicity in RL-34 cells

Methodology of Petri networks for simultaneous evaluation of the impact of different modifiers on the fluorescence of nucleotides from electron transport chain in isolated mitochondria and on the process of swelling

Genes and physical fitness