Wyniki wyszukiwania

1

Ole e 10, a 1,3-β-glucanase from olive pollen, co-localizes to callose during pollen tube growth

100%

Suarez C. G., Barral P., de Dios Alche J., Rodriguez R., Rodriguez-Garcia M. I.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

2

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Characterization of outer membrane proteins participating in iron transport in Pasteurella multocida serotype A3

80%

Puchalski A., Dec M., Wernicki A., Urban-Chmiel R., Gieral A.

Polish Journal of Veterinary Sciences

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2010

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tom 13

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nr 1

121-127

Iron-regulated outer membrane proteins (IROMPs) of P. multocida serotype A3, which function as receptors for complexes containing iron ions, are induced by iron deficiency in the bacterial growth environment. Analysis of an electrophoresis image of proteins isolated from bacteria grown on medium supplemented with 2,2'-dipyridyl revealed expression of 16 new proteins that were not noted in the case of the bacteria grown in standard conditions, with molecular weights from 30 to 160 kDa. Induction of IROMP expression occurred within 30 minutes after restricted iron conditions were established. In immunoblotting, distinct reactions were noted for proteins of molecular weight ranges of 25-49 kDa, 61-95 kDa, and 108-214 kDa. Proteins of the molecular weight of 68, 75 and 86 kDa were analysed using mass spectrometry and matched with the highest probability to proteins in the NCBI data base. Several dozen different proteins with similar amino acid sequences were matched to each sample.

3

Attaching a spin to a protein - site-directed spin labeling in structural biology

80%

Czogalla A., Pieciul A., Jezierski A., Sikorski A. F.

Acta Biochimica Polonica

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2007

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tom 54

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nr 2

Site-directed spin labeling and electron paramagnetic resonance spectroscopy have recently experienced an outburst of multiple applications in protein science. Numerous interesting strategies have been introduced for determining the structure of proteins and its conformational changes at the level of the backbone fold. Moreover, considerable technical development in the field makes the technique an attractive approach for the study of structure and dynamics of membrane proteins and large biological complexes at physiological conditions. This review focuses on a brief description of site-directed spin labeling-derived techniques in the context of their recent applications.

4

Complex analysis of genes involved in the inflammatory response: interleukin-1-induced differential transcriptome of cultured human hepatoma HepG2 cells

80%

Koj A., Jura J.

Acta Biochimica Polonica

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2003

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tom 50

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nr 3

The systemic inflammatory reaction (acute phase response) is induced by many noxious stimuli but in all cases the inflammatory cytokines, such as interleukin-1-beta (IL-1/ß) and interleukin-6 (IL-6), are involved. Liver cell response to inflammation manifested by a characteristic change in the profile of synthesized plasma proteins (acute phase proteins) has been extensively studied. Here we describe a model system of cultured human hepatoma HepG2 cells stimulated with IL-1/ ß to evaluate the transcriptome induced by this cytokine during 24 h of treatment. By using differential display analysis we found IL-1/ß-induced upregulation of several genes coding for cellular trafficking/motor proteins, proteins participating in the translation machinery or involved in posttranscription/posttranslation modifications, proteases, proteins involved in cellular metabolism, activity modulators, proteins of the cell cycle machinery and also some new proteins so far functionally not classified.

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Acta Biologica Cracoviensia. Series Botanica. Supplement

1 Polish Journal of Veterinary Sciences

1 Barral P.

1 Czogalla A.

1 Dec M.

1 Gieral A.

1 Jezierski A.

1 Jura J.

1 Koj A.

1 Pieciul A.

1 Puchalski A.

1 Rodriguez R.

1 Rodriguez-Garcia M. I.

1 Sikorski A. F.

1 Suarez C. G.

1 Urban-Chmiel R.

1 Wernicki A.

1 de Dios Alche J.

1 2010

1 2007

1 2005

1 2003

Ole e 10, a 1,3-β-glucanase from olive pollen, co-localizes to callose during pollen tube growth

Characterization of outer membrane proteins participating in iron transport in Pasteurella multocida serotype A3

Attaching a spin to a protein - site-directed spin labeling in structural biology

Complex analysis of genes involved in the inflammatory response: interleukin-1-induced differential transcriptome of cultured human hepatoma HepG2 cells