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Effects of homogenization of induced sputum by dithiothreitol on polymorphonuclear cells

100%
Van Overveld F. J., Demkow U., Gorecka D., Skopinska-Rozewska E., De Backer W. A., Zielinski J.
Journal of Physiology and Pharmacology. Supplement
|
2005
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tom 56
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nr 4
143-154
Induced sputum represents a useful and non-invasive tool to isolate different cells from the airways. Complete homogenization of sputum is important for dispersion of cells and is usually achieved by use of dithiothreitol (DTT). However, it is not known if DTT will influence the viability and functionality of cells obtained by induced sputum. In the present study, induced sputum was processed by DTT or by PBS treatment. The obtained neutrophils were compared with neutrophils obtained from peripheral blood and from bronchoalveolar lavage fluid (BAL). These isolated neutrophils were treated in a similar way as the sputum neutrophils with DTT or PBS. All isolated cells were used for chemiluminescence tests and for the measurement of elastase and myeloperoxidase release after stimulation with fMLP. The results showed that the maximum chemiluminescence response was always significantly lower after DTT treatment: blood, 16.68 ±1.89 vs. 2.62 ±0.43 mV, P<0.0001; sputum, 2.96 ±0.30 vs. 1.09 ±0.01 mV, P<0.01; BAL, 25.47 ±0.88 vs. 8.22±0.20 mV, P<0.0001. Both spontaneous and fMLP-induced release of elastase and myeloperoxidase (MPO) was in most cases enhanced after DTT-treatment (P-values range from 0.24 to <0.01). We conclude that the use of DTT to homogenize sputum for dispersion of cells is harmful to cell functions and these cells are hampered for the evaluation of their normal functional characteristics.
2

Effect of general and regional anesthesia on some neutrophil functions

100%
Ciepichal J., Kubler A.
Archivum Immunologiae et Therapiae Experimentalis
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1998
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tom 46
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nr 3
3

Regulation of neutrophil functions by elastase-generated IgG fragments

100%
Eckle I., Kolb G., Havemann K.
Archivum Immunologiae et Therapiae Experimentalis
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1992
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tom 40
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nr 1
4

Neutrophil function and apoptosis in patients with chronic hepatitis C treated with pegylated interferon alpha and ribavirin

80%
Jablonowska E., Wojcik K., Kur B., Lewkowicz P., Nocun M.
Archivum Immunologiae et Therapiae Experimentalis
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2012
|
tom 60
|
nr 1
5

The application of flow cytometric assay for evaluation of phagocytosis of neutrophils

80%
Zielinska M., Fenrych W.
Acta Biochimica Polonica
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1997
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tom 44
|
nr 1
Phagocytosis and the release of oxidative products generated by the respira­tory burst have been studied in vitro under the influence of non-steroidal anti-inflammatory drugs: naproxen and ibuprofen, using.phagocytes of periph­eral blood from healthy human donors. Phagocytosis wis monitored by flow cytometry in order to investigate the uptake of propvdium iodide-labelled bacteria (Staphylococcus aureus) by polymorphonuclear leucocytes. In addi­tion, the phagocytic capacity and percentage of killed bacteria was measured in isolated neutrophils using the Pantazis & Kniker method. It was found that naproxen and ibuprofen affect the phagocytic function and hydrogen peroxide production in the examined granulocytes.
6

Odporność: wrodzona i nabyta odpowiedź immunologiczna u krów w okresie przejściowym

61%
McKay S.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2015
|
tom 10
|
nr 3
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