Wyniki wyszukiwania

1

Depletion of guanine nucleotides in human neuroblastoma cell lines

100%

Agliano A. M., Gazzaniga P., Messina E., Micheli V., Spoto G., Giacomello A.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 3

2

Cytotoxicity of cyclopentenyl cytosine towards neuroblastoma cell line cells

88%

Bierau J., Van Gennip A. H., Van Kuilenburg A. B. P.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 3

3

Synergistic effects of amyloid peptides and lead on human neuroblastoma cells

51%

Suresh C., Johnson J., Mohan R., Chetty C. S.

Cellular and Molecular Biology Letters

|

2012

|

tom 17

|

nr 3

Aggregated amyloid peptides (AP), major components of senile plaques, have been considered to play a very important and crucial role in the development and neuro-pathogenesis of Alzheimer’s disease (AD). In the present in vitro, study the synergistic effects of Pb2+, a heavy metal, and AP on the human neuroblastoma SH-SY5Y cells were investigated. The cells treated with Pb2+ (0.01–10 μM) alone exhibited a significant decrease in viability and IC50 was 5 μM. A similar decrease in viability was also observed when the cells were exposed to AP, Aβ1–40 (20–120 μM) and Aβ25-35 (2.5–15 μM) for 48 hrs. The IC50 values were 60 μM and 7.5 μM for Aβ1–40 and Aβ25–35 respectively. To assess the synergistic effects the cells were exposed to IC50 of both AP and Pb2+, which resulted in further reduction of the viability. The study was extended to determine the lactate dehydrogenase (LDH) release to assess the cytotoxic effects, 8-isoprostane for extent of oxidative damage, COX 1 and 2 for inflammation related changes, p53 protein for DNA damage and protein kinases A and C for signal transduction. The data suggest that the toxic effects of AP were most potent in the presence of Pb2+, resulting in an aggravated clinical pathological condition. This could be attributed to the oxidative stress, inflammation neuronal apoptosis and an alteration in the activities of the signaling enzymes.

4

Modulation by testosterone of an endogenous hERG potassium channel current

51%

Ridley J. M., Shuba Y. M., James A. F., Hancox J. C.

Journal of Physiology and Pharmacology

|

2008

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tom 59

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nr 3

395-407

hERG (human ether-a-go-go-related gene) potassium (K+) channels are expressed in a range of tissue types including neuroblastoma cells and the heart, in which hERG K+ current is important for action potential repolarization. Whilst gender differences in cardiac repolarization and the QT interval of the cardiac electrocardiogram are well-established, comparatively little is known about regulation of hERG channels by sex hormones. In this study, whole-cell patch-clamp recordings were made at 37 °C from SH-SY5Y human neuroblastoma cells to investigate modulation of endogenous hERG K+ channel current (IhERG) by testosterone. Acutely applied testosterone at a physiologically relevant concentration (10 nM) produced a modest (~13-15 %) increase in IhERG amplitude, whilst a high concentration (1 µM) slightly decreased IhERG. The stimulatory effect of testosterone was inhibited by the androgen receptor antagonist flutamide (10 µM) and the PI-3 kinase inhibitor wortmannin (1 µM). Chronic (24 h) application of testosterone also augmented IhERG via flutamide-sensitive receptor activation, without modulation of the current's voltage-dependence. These results demonstrate for the first time that testosterone can stimulate hERG K+ channels via activation of classical androgen receptors and implicate PI-3 kinase in the acute response.

5

Lead-induced cell death of human neuroblastoma cells involves GSH deprivation

51%

Chetty C. S., Vemuri M. C., Campbell K., Suresh C.

Cellular and Molecular Biology Letters

|

2005

|

tom 10

|

nr 3

Ograniczanie wyników

4 Cellular and Molecular Biology Letters

1 Journal of Physiology and Pharmacology

2 Chetty C. S.

2 Suresh C.

1 Agliano A. M.

1 Bierau J.

1 Campbell K.

1 Gazzaniga P.

1 Giacomello A.

1 Hancox J. C.

1 James A. F.

1 Johnson J.

1 Messina E.

1 Micheli V.

1 Mohan R.

1 Ridley J. M.

1 Shuba Y. M.

1 Spoto G.

1 Van Gennip A. H.

1 Van Kuilenburg A. B. P.

1 Vemuri M. C.

1 2012

1 2008

1 2005

2 1999

Depletion of guanine nucleotides in human neuroblastoma cell lines

Cytotoxicity of cyclopentenyl cytosine towards neuroblastoma cell line cells

Synergistic effects of amyloid peptides and lead on human neuroblastoma cells

Modulation by testosterone of an endogenous hERG potassium channel current

Lead-induced cell death of human neuroblastoma cells involves GSH deprivation