This paper presents results of determination of the total content of arsenic, antimony and selenium in samples of surface water from selected lakes of Pszczewski Landscape Park. The concentrations of arsenic, antimony and selenium determined by the absorption atomic spectrometry method were up to 0.66 ng/ml, 0.62 ng/ml and 0.76 ng/ml, respectively, at the detection limits of 0.04 ng/ml for As, 0.04 ng/ml for Sb and 0.03 ng/ml for Se. The water samples studied revealed low concentrations of the elements of interest relative to the values obtained for the samples from other regions. The determined concentrations of arsenic, antimony and selenium can be interpreted as natural.