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Isolation, cloning and characterisation of motifs containing [GA-TC] repeats isolated from vetch, Vicia bithynica

100%

Sakowicz T., Bowater R., Parniewski P.

Cellular and Molecular Biology Letters

2004

tom 09

nr 3

Microsatellites are widely distributed in plant genomes and comprise unstable regions that undergo mutational changes at rates much greater than that observed for non-repetitive sequences. They demonstrate intrinsic genetic instability, manifested as frequent length changes due to insertions or deletions of repeat units. Detailed analysis of 1600 clones containing genomic sequences of Vicia bithynica revealed the presence of microsatellite repeats in its genome. Based on the screening of a partial DNA library of plasmids, 13 clones harbouring (GA/TC)n tracts of various lengths of repeated motif were identified for further analysis of their internal sequence organization. Sequence analyses revealed the precise length, number of repeats, interruptions within tracts, as well as sequence composition flanking the repeat motifs. Representative plasmids containing different lengths of (GA/TC)n embedded in their original flanking sequence were used to investigate the genetic stability of the repeats. In the study presented herein, we employed a well characterised and tractable bacterial genetic system. Recultivations of Escherichia coli harbouring plasmids containing (GA/TC)n inserts demonstrated that the genetic instability of (GA/TC)n microsatellites depends highly on their length (number of repeats). These observations are in agreement with similar studies performed on repetitive sequences from humans and other organisms.

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Mutational changes in Delphinium malabaricum (Huth.) Munz.: a potential ornamental plant

84%

Kolar F. R., Ghatge S. R., Nimbalkar M. S., Dixit G. B.

Journal of Horticultural Research

2015

tom 23

nr 2

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Journal of Horticultural Research

1 Bowater R.

1 Dixit G. B.

1 Ghatge S. R.

1 Kolar F. R.

1 Nimbalkar M. S.

1 Parniewski P.

1 Sakowicz T.

1 2015

1 2004

Wyniki wyszukiwania

Isolation, cloning and characterisation of motifs containing [GA-TC] repeats isolated from vetch, Vicia bithynica

Mutational changes in Delphinium malabaricum (Huth.) Munz.: a potential ornamental plant