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Improvement of Aureobasidium pullulans A.P.-3 for pullulan biosynthesis by assiciated mutagenesis

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Gniewosz M., Sobczak E., Wojciechowska D., Kuthan-Styczen J.

Polish Journal of Food and Nutrition Sciences

1999

tom 08

nr 2

235-243

The strain Aureobasidium pullulans A.p.-3 was subjected to mutagenesis to improve its ability to pullulan biosynthesis. The mutagenesis of 18-h old culture was carried out in two variants using ethylen imine (EI) and ultraviolet (UV) radiation at the EI concentration and UV exposure times of 2.0 mg/mL and 1.5 min (variant I), and 2.0 mg/mL and 2.0 min (variant II). Among 240 cultures examined, the positive mutants made 26% for variant I and 13% for variant II. Two best black mutants Dy-17 and Dx-30 synthesized the polysaccharide with the yields greater than those of parent strain by 24 and 21%, respectively. White mutant was also obtained, the pullulan yield for which was on the level as for strain A.p.-3, but free from melanin contaminants. Acquired characteristic of black mutants was found stable when re-examined after 3- and 6-month storage at 4°C. The kinetics of pullulan biosynthesis by black mutants indicated that the pullulan production was intensified beginning from the second day of culturing, as compared to strain A.p.-3.

Ograniczanie wyników

1 Polish Journal of Food and Nutrition Sciences

1 Gniewosz M.

1 Kuthan-Styczen J.

1 Sobczak E.

1 Wojciechowska D.

1 1999

Wyniki wyszukiwania

Improvement of Aureobasidium pullulans A.P.-3 for pullulan biosynthesis by assiciated mutagenesis