Studies were carried out on the effect of various methods of storing municipal refuse upon the survival and passage of saprophytic microorganisms (TVC, Gram positive bacteria, Gram negative bacteria, thermotolerants, yeasts), bacteria indicative of sanitary conditions (TC, FC, E. coli, FS, enterococci, Clostridium perfringens) and opportunistic pathogens (hemolytic streptococci, staphylococci, in this Staphylococcus aureus) into leachates. Studies were made from June to October using PCV columns 105 mm in diameter and 1000 mm high, in 5 combinations: 1) permeable bottom, 2) bottom sealed with a 150 mm layer of clay, 3) bottom sealed with a 150 mm layer of fly ash, 4) permeable bottom but municipal refuse mixed with fly ash, and 5) bottom sealed with a 75 mm layer of fly ash. Bacteria counts in fresh refuse were typical of untreated municipal sewage. With the exception of thermotolerants and hemolytic streptococci, bacteria and yeast counts decreased after 143 days, although to a varying extent. Leachates from municipal refuse stored in columns in particular experimental combinations usually contained the highest numbers of microorganisms during the first 35 days. Later on their counts decreased. On the average, the lowest numbers of the microorganisms were leached from the columns sealed with a 150 mm layer of fly ash, while the highest numbers were observed in the case of columns with permeable bottom and those sealed with a 150 mm layer of clay.
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