Wyniki wyszukiwania

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Genomic distribution of genes encoding 68 cytoplasmic ribosomal protein families in rice

100%

Agarwal S. M., Jain M., Grover A.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 4A

Completion of rice genome sequencing has necessitated identification of transcripts encoded by the genome and their corresponding functions. In the present study we have catalogued cytoplasmic ribosomal protein complement of rice genome corresponding to 68 families. Mining TIGR (The Institute for Genomic Research) rice database and unigene sequences available from National Centre for Biotechnology Information (NCBI) produced a non-redundant set of these sequences. This resulted in identification of 209 candidate r-proteins of which 22 have not been reported previously. The number of genes per family ranges from 1 to 8 distributed throughout the genome with maximum occurrence at chromosome 7. Mapping of r-proteins on BAC clones revealed several small clusters of genes. Unigene sequences correspondtng to most of the reported r-proteins were identified indicating these genes are being expressed.

2

AMPD1: ancient mutations provide insight into the pathogenesis of modern day disorders

100%

Loh E., Oefner P., Holmes E.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

3

The multigene family of fatty acid-binding proteins [FABPs]: Function, structure and polymorphism

84%

Chmurzynska A.

Journal of Applied Genetics

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2006

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tom 47

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nr 1

Fatty acid-binding proteins (FABPs) are members of the superfamily of lipid-binding proteins (LBP). So far 9 different FABPs, with tissue-specific distribution, have been identified: L (liver), I (intestinal), H (muscle and heart), A (adipocyte), E (epidermal), Il (ileal), B (brain), M (myelin) and T (testis). The primary role of all the FABP family members is regulation of fatty acid uptake and intracellular transport. The structure of all FABPs is similar- the basic motif characterizing these proteins is β-barrel, and a single ligand (e.g. a fatty acid, cholesterol, or retinoid) is bound in its internal water-filled cavity. Despite the wide variance in the protein sequence, the gene structure is identical. The FABP genes consist of 4 exons and 3 introns and a few of them are located in the same chromosomal region. For example, A-FABP, E-FABP and M-FABP create a gene cluster. Because of their physiological properties some FABP genes were tested in order to identify mutations altering lipid metabolism. Furthermore, the porcine A-FABP and H-FABP were studied as candidate genes with major effect on/fatness traits.

4

Structural and functional characteristics of two novel members of pathogenesis-related multigene family of class from yellow lupine

67%

Handschuh L., Femiak I., Kasperska A., Figlerowicz M., Sikorski M. M.

Acta Biochimica Polonica

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2007

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tom 54

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nr 4

783-796

PR-10 proteins (pathogensis-related), ubiquitous within the plant kingdom, are usually encoded by multigene families. To date we have identified 10 homologous pr-10 genes in a yellow lupine cDNA library. Here, the structure and expression of two newly identified yellow lupine pr-10 genes (LlYpr10-2b and LlYpr10-2f) are presented. Many potential regulatory sites were found in both gene promoters including common ones as well as those unique for each gene. However, promoter deletion analysis in transgenic tobacco plants revealed similar patterns of reporter gene (gus) expression. Shortened fragments of both gene promoters studied caused high GUS activity in leaves (along vascular bundles), stamen stigma, anthers and pollen grains. When conjugated with longer LlYpr-10.2 promoter fragments, GUS was additionally present in petal edges. Only a long fragment of the LlYpr10-2b gene promoter caused GUS expression in the stem. In yellow lupine the pr-10.2 genes are present in all studied organs, but their level of expression depends on the stage of development and is affected by wounding, oxidative stress and salicylic acid treatment. Silencing of the Llpr-10.2b gene in 4-week-old yellow lupine plants did not lead to any visible symptoms, which suggests that the function of the silenced gene is supplemented by its close homologues, still present in the studied plants.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Acta Physiologiae Plantarum

1 Cellular and Molecular Biology Letters

1 Journal of Applied Genetics

1 Agarwal S. M.

1 Chmurzynska A.

1 Femiak I.

1 Figlerowicz M.

1 Grover A.

1 Handschuh L.

1 Holmes E.

1 Jain M.

1 Kasperska A.

1 Loh E.

1 Oefner P.

1 Sikorski M. M.

1 2007

1 2006

1 2005

1 1999

Genomic distribution of genes encoding 68 cytoplasmic ribosomal protein families in rice

AMPD1: ancient mutations provide insight into the pathogenesis of modern day disorders

The multigene family of fatty acid-binding proteins [FABPs]: Function, structure and polymorphism

Structural and functional characteristics of two novel members of pathogenesis-related multigene family of class from yellow lupine