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Pulsed-field gel electrophoresis as a molecular tool for characterizing genomes of certain food-borne bacterial isolates - a review

100%

Pal P.

International Letters of Natural Sciences

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2015

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tom 02

The evolutionary transition from phenotypic to molecular analysis of infectious disease in bacterial epidemiology led to the search for suitable approaches to ascertain genomic relatedness or heterogeneity between bacterial clinical isolates. Pulsed-field gel electrophoresis (PFGE) technique was developed for separating and analyzing long DNA fragments of several megabases in alternating electric field. Comparison of electrophoresis profiles of restriction enzyme-digested genomic DNA from bacterial isolates has proved to be a useful epidemiological tool for genetic discrimination of bacterial strains, detection of genetic relatedness, to locate the source of outbreak and to monitor the spread of the microorganisms in endemic zones. PFGE is considered as a gold standard method for typing of bacterial isolates because of the remarkable endurance of this technique as a typing method for the last 20 years in molecular epidemiology. In this current review the pros and cons of PFGE use in current molecular microbiological research are explored in the context of determination of genome organization of certain food-borne bacterial isolates causing infectious diseases in human beings.

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Characterization of Staphylococcus aureus strains isolated from cystic fibrosis patients by conventional and molecular typing

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Sadowska B., Cochard T., Poutrel B., Pytlos M., Bonar A., Gatkowska J., Rudnicka W., Polak J., Bielecki S., Siwinska-Golebiowska H., Rozalska B.

Acta Microbiologica Polonica

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2001

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tom 50

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nr 3-4

251-261

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Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

84%

Karakulska J., Pobucewicz A., Nawrotek P., Muszynska M., Furowicz A. J., Czernomysy-Furowicz D.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 2

The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.

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Molecular typing, biodiversity, and biological control of endophytic fungi of Triticum aestivum L. against phytopathogenic fungi of wheat

84%

Hassanein N. M., El-Gendy M. M. A. A., Abdelhameed N. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2020

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tom 101

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nr 4

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Endogenous or exogenous origin of vaginal candidiasis in Polish women?

84%

Mnichowska-Polanowska M., Wojciechowska-Koszko I., Klimowicz B., Szymaniak L., Krasnodebska-Szponder B., Szych Z., Giedrys-Kalemba S.

Polish Journal of Microbiology

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2013

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tom 62

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nr 3

Vaginal candidiasis is a common problem of clinical practice. Many studies have been conducted to explain its origin but only a few have included Polish women. The aim of the study was to determine the prevalence and similarity of oral, anal and vaginal Candida albicans strains isolated from Polish women with vaginal candidiasis. The study involved 20 from 37 recruited women. Swab samples were collected from their vagina, anus, and oral cavity at two-month intervals. All the women were treated with nystatin. Yeast were recovered and identified by the germ-tube test, API /Vitek system, typed by API ZYM and RAPD-PCR. Chi-square test was used to analyze the data. A total of 170 Candida albicans isolates were recovered from 180 samples collected 3 times from 3 sites of 20 women. Positive yeast vaginal cultures were found in all patients before administration of nystatin. Vaginal yeast recovery rate was decreased statistically significant in both follow-up visits (p =0.001; p =0.003). The same and different genotypes/biotypes were found concomitantly in a few body sites and/ or repeatedly at time interval from the same body site. The results support the concept of dynamic exchange of yeast within one woman and endogenous or exogenous origin of vaginal candidiasis.

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A two-step strategy for molecular typing of multidrug-resistant Mycobacterium tuberculosis clinical isolates from Poland

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Jagielski T., Augustynowicz-Kopec E., Pawlik K., Dziadek J., Zwolska Z., Bielecki J.

Polish Journal of Microbiology

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2011

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tom 60

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nr 3

Tuberculosis (TB) continues to be one of the most challenging public health problems in the world. An important contributor to the global burden of the disease is the emergence and spread of drug-resistant and particularly multidrug-resistant Mycobacterium tuberculosis strains (MDR), defined as being resistant to at least isoniazid and rifampicin. In recent years, the introduction of different DNA-based molecular typing methods has substantially improved the knowledge of the epidemiology of TB. The purpose of this study was to employ a combination of two PCR-based genotyping methods, namely spoligotyping and IS6110-Mtb1/Mtb2 PCR to investigate the clonal relatedness of MDR M. tuberculosis clinical isolates recovered from pulmonary TB patients from Poland. Among the 50 isolates examined, 28 (56%) were clustered by spoligotyping, whereas IS6110-Mtb1/Mtb2 PCR resulted in 16 (32%) clustered isolates. The isolates that clustered in both typing methods were assumed to be clonally related. A two-step strategy consisting of spoligotyping as a first-line test, performed on the entire pool of isolates, and IS6110-Mtb1/Mtb2 PCR typing as a confirmatory subtyping method, performed only within spoligotype-defined clusters, is an efficient approach for determining clonal relatedness among M. tuberculosis clinical isolates.

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Typowanie molekularne szczepow Campylobacter przy uzyciu technik opartych na reakcji lancuchowej polimerazy

51%

Wieczorek K., Osek J.

Acta Scientiarum Polonorum. Medicina Veterinaria

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2008

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tom 07

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