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Haploidy in early bovine embryos produced in vitro

100%

Lechniak D.

Journal of Applied Genetics

1995

tom 36

nr 4

Early bovine embryos (two - to sixteen blastomeres) produced in vitro were cytogenetically analysed to determine the incidence of haploidy. Follicular oocytes were matured in vitro and inseminated with sperm prepared using the swim up method. After 2-3 days of culture, chromosome slides were prepared according to the air-drying technique and stained with Giemsa. Altogether 202 embryos produced metaphase spreads. Of these 4.5% were pure haploid embryos and 5.5% displayed a haploid cell line within mosaic embryos (n/2n and n/3n). The occurrence of haploid embryos observed in this study was compared to results of other studies and a possible origin of haploidy was discussed.

Ograniczanie wyników

1 Journal of Applied Genetics

1 Lechniak D.

1 1995

Wyniki wyszukiwania

Haploidy in early bovine embryos produced in vitro