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Influence of He-Ne laser irradiation and cadmium and lead on changes in cell cycles at Zea mays L.

100%

Rozanowski B., Mozdzen K., Wojcik J., Nosalski R., Zandi P., Wang Y.

Agronomy Science

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2020

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tom 75

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nr 1

2

Isolation and antimitotic activity of polysaccharides from fruit bodies of Xerocomus badius [Fr.] Kuhn. ex Gilib.

100%

Wegiel J., Konska G., Guillot J., Muszynska B., Bohatier J., Kohlmunzer S.

Acta Biologica Cracoviensia. Series Botanica

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2001

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tom 43

Two polysaccharide fractions were isolated from Xerocomus badius fruit bodies with a dry weight yield of 0.41% (Polysaccharide fraction A) and 0.89% (Polysaccharide fraction B). Their sugar content was 99.5% and 98.7%, respectively, Polysaccharide fraction A was composed of glucose, while Polysaccharide fraction B contained mainly glucose and mannose. Biological activity testing using the Allium test showed mitostatic and mitodepressant activity for both fractions. Polysaccharide fraction A exhibited a mitostatic action at a concentration of 0.5%, while Polysaccharide fraction B showed such activity at 0.5% and 0.25% concentrations after 24 h and 48 h. Polysaccharide fraction B exerted a direct influence on cell division of the tested organism in the Tetrahymena test.

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Influence of N,N-bis[3-aminopropylo]dodecyloamine on the ultrastructure of nuclei in Aspergillus niger mycelium and on cell proliferation and mitotic disturbances in Allium cepa L. root meristem

100%

Gabara B., Kalwinek J., Kozirog A., Zakowska Z., Brycki B.

Acta Biologica Cracoviensia. Series Botanica

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2006

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tom 48

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nr 1

Hyphae growing for 1 h in the presence of 0.05% and 0.1% N,N-bis(3-aminopropylo)dodecyloamine (APDA) showed malformations in the nuclear structure, including changes in shape, budding, progressive disappearance of the nucleolus and chromatin, and damage of the nuclear envelope. The Allium test revealed that at both concentrations used (0.005%, 0.01%) the tested substance lowered the frequency of dividing cells and induced mitotic abnormalities such as c-metaphases, anaphase-telophase bridges and sticky chromosomes, lagging chromosomes, micronuclei, binucleate cells, budding nuclei and partial extrusion of the nucleolus from the nucleus. These data indicate that APDA has cytotoxic, mitodepressive and turbogenic effects, especially at the higher dose. The results showed that APDA may be safely used as a microbicidal agent to destroy microorganisms developing on experimental material.

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Cell cycle synchronization in the root meristem of Allium cepa L.

100%

Woloszyn M., Golczyk H., Joachimiak A.

Acta Biologica Cracoviensia. Series Botanica

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2001

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tom 43

A high level of cell cycle synchronization in Allium cepa was achieved using a 24 h hydroxyurea block (1.25 mM) followed by 20 h of recovery (without hydroxyurea) and a subsequent 4 h treatment with saturated α-bromonaphthalene solution. This procedure was very effective in producing a synchronously dividing cell population with a 76% mean mitotic index and a 30% frequency of metaphase stages. After release from hydroxyurea arrest, large fractions of cells synchronously entered successive phases: G1, S, and G2. The calculated duration of the cell cycle, 13 h, is in general accordance with previous reports. A detailed protocol for obtaining a high mitotic index and a large portion of cells in a particular cycle phase (G1, S, G2) was prepared.

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Clastogenic effects of antioxidant ethoxyquin observed in Vicia faba L. ssp. minor root meristem cells

100%

Kontek R., Osiecka R., Matlawska-Wasowska K., Blaszczyk A.

Acta Toxicologica

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2006

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tom 14

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nr 1-2

6

Mitodepressive and cytotoxic effects of short-term exposure to relatively small doses of pendimethalin evaluated by Allium test

80%

Tylicki A., Kowalska-Wochna E., Oscilowicz A., Ilasz R.

Polish Journal of Environmental Studies

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2010

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tom 19

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nr 1

193-199

Pendimethalin is an extensively used dinitroaniline herbicide. The half-life of pendimethalin in soil can be long, and accumulation of this herbicide in the environment is probable. Therefore, we investigated the influence of pendimethalin (0.033, 0.066, 0.099, 0.132 and 0.264 g/L) on cell division during short-term treatment (24 and 48 hours) using Allium test. We observed inhibition of root elongation after 48 hours of incubation with pendimethalin. This effect was caused by the inhibition of mitoses varying from 1/3 to 1/2 in the case of 0.033, 0.066 and 0.099 g/L of pendimethalin, and almost complete restriction of mitoses under higher concentrations. Pendimethalin caused mitotic disturbances (c-metaphases, anaphasal and telophasal chromosome bridges, multipolar anaphases) and interphase abnormalities (micronuclei, multinuclear cells). This effect was irreversible during a 48-hour postincubation in water. Mitotic disturbances were caused by abnormalities in the organization of the tubulin cytoskeleton. Results suggest that even small amounts of pendimethalin can be a danger for dividing cells and embryos.

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Chromosome aberrations, sister chromatid exchanges [SCE] and cell division kinetics in human lymphocytes exposed in vitro to purified trypsin inhibitor from Ascaris

80%

Blaszkowska J., Bratkowska A., Lopaczynska D., Strozynski H., Ferenc T.

Journal of Applied Genetics

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2004

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tom 45

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nr 2

Ograniczanie wyników

Influence of He-Ne laser irradiation and cadmium and lead on changes in cell cycles at Zea mays L.

Isolation and antimitotic activity of polysaccharides from fruit bodies of Xerocomus badius [Fr.] Kuhn. ex Gilib.

Influence of N,N-bis[3-aminopropylo]dodecyloamine on the ultrastructure of nuclei in Aspergillus niger mycelium and on cell proliferation and mitotic disturbances in Allium cepa L. root meristem

Cell cycle synchronization in the root meristem of Allium cepa L.

Clastogenic effects of antioxidant ethoxyquin observed in Vicia faba L. ssp. minor root meristem cells

Mitodepressive and cytotoxic effects of short-term exposure to relatively small doses of pendimethalin evaluated by Allium test

Chromosome aberrations, sister chromatid exchanges [SCE] and cell division kinetics in human lymphocytes exposed in vitro to purified trypsin inhibitor from Ascaris