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The studies aimed at identification of neoplastic cells at the S phase of mitotic cycle in mammary gland adenocarcinomas of bitches. The material was sampled from bitches of various races, aging 6 to 12 years, in which the mammary gland tumours developed spontaneously. The tumours were verified histopathologically and, then, immunohistochemical reactions were performed in order to detect cells which had incorporated BrdU (bromodeoxyuridine), contained Ki-67 or PCNA antigen. The histological preparations were photographed and obtained pictures were subjected to computer-assisted image analysis using Axiophot microscope (Carl Zeiss) coupled to a computer and the MultiScaneBase V 8.08 software, working under Windows. Fifty percent of sections from mammary gland adenocarcinomas demonstrated BrdU labelling index of 4-5%, 40% of 1-3%, while in the remaining 10% of examined tumours no BrdU incorporation could be demonstrated. No evident relationship could be detected between the presence of BrdU incorporation and Ki-67 or PCNA antigen presence but a significant correlation was demonstrated between the expression of Ki-67 and PCNA.
Embryo sac development in reciprocal crosses of winter, hexaploid: wheat (Triticum aestivum L. cultivar ‘Liwilla’) and triticale (xTriticosecale Wittm. strain CHD 464) was examined during the first five days after pollination. Double fertilization was found in both crosses. Embryos developed regularly, slower however in the wheat ♀ x triticale ♂ cross. Aberrant, polyploid (up to 24n) endosperm nuclei were formed through disturbed mitotic cycles in both crosses. They occurred more frequently in the wheat ♀ x x triticale ♂ cross. Also, an early desynchronization of nuclear divisions and an uneven distribution of endosperm nuclei were found in the latter cross. They were accompanied with delayed or not complete cellularization of endosperm and its degradation in some regions. These abnormalities are the possible cause of the severe kernel sterility in the wheat ♀ x triticale ♂ cross. In contrast, the reciprocal cross produced shrivelled but mostly viable kernels.
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