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1

DNA fingerprinting analysis of rabbits from lines divergently selected for high and low open-field activity

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Sacharczuk M., Jezierski T., Daniewski W., Gorecka A., Parada R., Swiergiel A. H., Jaszczak K.
Animal Science Papers and Reports
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2005
|
tom 23
|
nr 2
The aim of the study was to analyse whether a successful divergent selection for open-field activity (OFA) in rabbits has resulted in differences in DNA fingerprinting pattern and genetic parametres of diversity. We also scanned DNA fingerprinting profiles for searching minisatellite alleles potentially linked to genes determining the trait under selection. Rabbits derived from the 8th generation of the lines selected for high (H) or low (L) locomotor OFA were profiled for DNA fingerprinting using HinfI enzyme and 33.6 multilocus probe. The H and L lines represent different, i.e. active and passive coping strategies in a novel situation.Selection for H or L locomotor OFA did not affect significantly the molecular parametres of genetic diversity. However, the analysis of band patterns for individual and pooled DNA fingerprints revealed a specific band for the L line at 15 kbp. No specific bands for the H line were detected. The results presented provide evidence of a possible linkage between minisatellites and OFA in rabbits and demonstrate that studies on the H and L lines may give rise to a new strategy in animal breeding and selection for traits related to animal welfare (locomotion and fear-related emotional behavior) and to the study of genetic background of hyperactivity disorders, e.g. ADHD.
2

DNA fingerprinting of chicken lines divergently selected for high or low incidence of skeletal malformations

100%
Sacharczuk M., Parada R., Jaszczak K.
Animal Science Papers and Reports
|
2005
|
tom 23
|
nr 2
Genetic analysis was conducted in chicken belonging to lines divergently selected for high or low frequency of skeletal defects (line H and L, respectively). The analysis was based on the DNA fingerprinting technique, using enzyme HinfI and Jeffrey’s 33.6 probe. Principal aims were: 1) to determine the effects of selection on the genetic variation within and between lines, and 2) to search for the presence of line-specific minisatellite alleles (as bands in the DNA fingerprinting pattern) which could be used as markers for the predisposition to skeletal defects. DNA fingerprinting pattern (DFP) displayed differences in position of bands, some of them appearing characteristic only for one out of two chicken lines studied.
3

DNA fingerprinting patterns in two lines of mice divergently selected for high and low swim stress-induced analgesia

100%
Sacharczuk M., Sadowski B., Parada R., Swiergiel A. H., Jaszczak K.
Animal Science Papers and Reports
|
2005
|
tom 23
|
nr 2
DNA fingerprinting patterns (DFPs) obtained by multi-locus probes have been shown to be useful in assessing genetic variation and genetic distances in laboratory animals. Using this method DFP profiles were analysed of mice belonging to lines divergently selected over 56 generations for high (HA) and low (LA) swim stress-induced analgesia. Apart from nociception-related traits the HA mice displayed, as compared to the LA animals, higher emotionality in various behavioural tests, and higher degree of hypothermia when subjected to a hypothermic challenge. In earlier study the authors showed that HA mice appeared to be more susceptible to the mutagenic effect of whole-body ă-radiation and mitomycin C injection. Lower nucleolar organizer regions (NOR) activity was also observed. In the present study the use of DFP method demonstrated that selection for magnitude of swim stress-induced analgesia differentiated the parental outbred population into two distinct genotypem characterized by specific minisatellite sequences for each line that may be genetic markers for particular physiological and neuro-behavioural traits. It is concluded that the selection altered the frequencies of minisatellites which are linked to genes determining susceptibility to stress, resulting in differentiation of stress-related traits.
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