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Tree species differ in litter quality and belowground biomass, thereby exerting species-specific impact on soil properties and microbial biomass. A study was conducted to find out the comparative effects of Podocarpus falcatus and Croton macrostachys on basic soil characteristics and microbial biomass, in the Munessa forest, Ethiopia. Four experimental plots under the canopies the respected tree species (two from each) were established for sample collection. From these plots, soil samples were collected from a depth 0-10 cm and 10-25 cm. The results showed that, from the depth 0-10 cm, concentration of organic carbon (C) and nitrogen (N) was larger under C. macrostachys and from the depth 10-25 cm these values were greater under P. falcatus. There was significant difference (p < 0.05) in cation exchange capacity being larger under C. macrostachys. There were no differences in microbial composition between the plots. However, the total phospholipid fatty acids (PLFA) concentration as an entry for microbial biomass determination tended to be significantly larger in soil under Podocarpus plots (382.7 ± 60.9 nmol PLFA g⁻¹ dry soil) vs. 262.2 ± 32.8 nmol PLFA g⁻¹ dry soil (Croton plots). The varying impacts of tree species on soil characteristics and microbial biomass may be partly explained by differences in functional traits related to life-history strategy of the respected species.
Our study aimed to determine the effects of conventional diesel fuel modification with biodiesel addition on the activity of soil microbiota. Diesel fuel, biodiesel and their mixture in a concentration of 5% (w/w) were introduced into soils – light loamy silty sand and light silty loam. Based on the obtained results, reduction in the content of live microbial biomass was found, irrespective of the soil type and contamination. Despite the introduction of biodiesel into soils, inhibition was observed in all examined treatments throughout the entire incubation.
Signature lipid biomarker (SLB) analysis is a useful tool for identifying microorganisms and character­izing microbial communities in natural systems. Specific fatty acids, especially phospholipids (PLFA), are es­sential membrane components, make up a relatively constant proportion of the microorganisms under natural conditions and their patterns provide insight into the bacterial and fungal community structure and biomass. This method is based on direct extraction of fatty acids from cultured bacteria or environmental samples and determining the isolated methyl ester fatty acids (FAME) using gas chromatography (GC). Several PLFAs are useful markers for the detection of the specific groups, and whole cell fatty acid analysis is used for routine identification of microbial species. The fatty acid analysis has been successfully applied for the characteriza­tion of microbial communities from agricultural soils, from sites contaminated with heavy metals, aromatic compounds, alkaline dust, acid rain and from other diverse habitats.
Soil chemistry, soil microbial biomass and mineralization rate of organic matter (OM) were estimated on plots, which were set up in Scots pine and Norway spruce forests in southern Poland. The aim of the study was to assess the relations between soil microbial biomass and chemical parameters associated with soil fertility. The size of microbial biomass was found to be significantly correlated with chemical parameters reflecting soil nutritional status (content of base cations, cation exchange capacity, base saturation) and organic matter quality (Corg to macronutrients ratios). In addition, soil microbial biomass appeared to be strongly correlated with OM mineralization rate. These findings point out a distinct relationship between soil fertility and soil microbial biomass, suggesting that microbial biomass measurements provide a valid estimate of soil quality.
The objective of this study was to compare chemical and microbial properties of sandy mine soils under young Scots pine, silver birch, and mixed pine-birch forest stands. The measured properties included the contents of organic C (Corg) and total N (Nt), the Corg-to-Nt ratio, pH, microbial biomass, basal respiration, and activities of dehydrogenase, acid phosphomonoesterase, and urease. Community level physiological profiles (CLPPs) of soil bacteria were determined with BiologR test and genetic profiles with the DGGE method. Scots pine and silver birch did not affect the Corg and Nt contents in the studied mine soils. The soil under birch contained larger and more active microbial biomass than the soil under pine. Under the mixed stand, most of the microbial properties were intermediate between the pine and the birch stand. The DGGE profiling indicated different composition of soil bacteria under the birch stand compared to the other stands. Differences in CLPPs were less pronounced, probably due to functional redundancy of soil bacteria.
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The effect of tillage intensity on changes of microbiological activity and content of particulate organic matter in soil under winter wheat duirng 3 years was studied. Microbial response related to the tillage-induced changes in soil determined on the content of biomass C and N, the rate of CO2 evolution, B/F ratio, the activity of dehydrogenases, acid and alkaline phosphatases, soil C/N ratio and microbial biomass C/N ratio confirmed the high sensitivity of soil microbial populations to the tillage system applied. After three year studies, the direct sowing system enhanced the increase of labile fraction of organic matter content in soil. There were no significant changes in the labile fraction quantity observed in soil under conventional tillage. Similar response related to the tillage intensity was observed in particulate organic matter quantities expressed as a percentage of total organic matter in soil. A high correlation coefficients calculated between contents of soil microbial biomass C and N, particulate organic matter and potentiallymineralizable N, and the obtained yields of winter wheat grown on experimental fields indicated on a high importance of biological quality of status of soil for agricultural crop production.
The aim of this study was to assess initial effects of warming on the plant growth, soil nutrient contents, microbial biomass and enzyme activities of two most widespread ecosystem types: swamp meadow (deep soil, high water content) and alpine meadow (poor soil, low water content), in the hinterland of the Qinghai-Tibet Plateau (altitude 4600–4800 m a.s.l.) The temperature of air and upper soil layer was passively enhanced using open-top chambers (OTCs) (50 cm high with 60 cm at opening and constructed of 6 mm thick translucent synthetic glass) from 2006 to 2008. The use of OTCs clearly raised temperature and decreased soil moisture. In warmed plots, monthly mean air temperature was increased by 2.10oC and 2.28oC, soil moisture of 20 cm soil layer was decreased by 2.5% and 3.9% in alpine meadow and swamp meadow, respectively. Plant biomass significantly increased by 31% in alpine meadow and 67% in swamp meadow. Warming also affected soil microbial biomass C and N at both meadows. In swamp meadow, warming caused the decrease of soil organic carbon and total nitrogen in 0–5 cm layer and an increase in 5–20 cm. While in alpine meadow, these soil parameters increased in 0–5 cm layer and decreased in 5–20 cm layer. The effects of warming on enzyme activities differed depending on the enzyme and the meadow ecosystem. In general, enzyme activities were higher in the upper soil layers (0–5 cm) than in the lower soil layers (5–20 cm). The experiment results exhibited that warming improved the soil biochemical and microbiological conditions in high- mountain meadows, at least in the short term.
Studies were carried out in a 8 years old shelterbelt planted across croplands (D. Chłapowski Landscape Park, region of Wielkopolska, western Poland) and in the easterly adjacent field. The effect of the shelterbelt on: soil organic matter content, soil respiration, microbial biomass, dehydrogenase activity, mineralization potential of N and total number of bacteria and fungi in the upper soil layer was analysed. Samples were collected along parallel sites situated in the central part of the strip (S), in the wood edge (Es), field edge (Ef) and in the field 10 m (F10) and 50 m (F50) away from the strip. All studied microbial parameters, except for plate counted total numbers of bacteria and fungi, showed a high and significant correlation with soil respiration and microbial biomass. The highest values were found in the wood soil, lowest in the field soil and intermediate in the field edge. Marked vertical differences between layers 0-3 and 3-10 cm were noticed on sites S, Es and Ef while stratification in the field was visible not earlier than 11 months after ploughing. Some parameters: soil organic matter contents, dehydrogenase activity, microbial biomass estimated by fumigation-extraction method and mineralization potential of N, showed a regular pattern along the gradient from the shelter-belt to the middle of the field. The study suggests a favourable effect of a shelterbelt on organic matter content in the adjacent field. This effect may increase with age of the wood strip.
Changes in densities of microorganisms were analysed in Cognetia sphagnetorum Vejd. cultures at normal and low numbers of microorganisms. Different abundance of microorganisms was achieved by using captan and oxytetracycline. During 14 weeks, the following variables were measured: total numbers of bacteria and fungi by plate method, the biomass of microorganisms by PLFA method, content of ions, and activity of acid and alkaline phosphatase and urease. Numbers of enchytraeids increased with numbers of fungi. The presence of animals reduced the activity of soil enzymes and the content of Cl, SO₄, Na, Ca, and Mg ions in soil leacheates. An increase in the content of N-NO₃ and N-NH₄ was not significant.
Studies on the comparison of microbial and biochemical characteristics of soils were based on a field experiment established in 1994 at the IUNG Experimental Station in Osiny on soil (loamy sand) belonging to complex IV. In this experiment spring barley and winter wheat were grown in three crop production systems: ecological, integrated and conventional. Soil under wheat were analysed in 1996, 1997 and 1998, and under barley in 1997 and 1998. Microbial biomass, numbers of bacteria and fungi, soil respiration and enzymes (dehydrogenase, phosphatases) activity were generally higher in the soil under the tested cereals grown in the ecological system than in the soil under these crops grown in the conventional or integrated system. These differences were detected during all three growing seasons, indicating long-lasting changes in the biological characteristics of the studied soil.
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