The aim of the study was to evaluate the TRAP assay method of measuring telomerase activity in canine skin neoplasms. Telomerase activity was measured in 57 samples of tumor tissues from operated dogs. The samples were also subjected to histopathology investigations. Telomerase activity was measured using the Telo TAGGG Telomerase PCR ELISA diagnostic kit as well as ROCHE . photometric enzyme immunoassay for quantitative determination of telomerase activity, utilizing the telomere repeat amplification protocol (TRAP). The repetition, sensitivity and specificity of telomerase activity measurements were determined. TRAP assay performed in dogs has high repetition rate within high activity, and lower within small activity. This method is 100% sensitive and 34% specific.
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