The aim ofthe study was to determine the effects of illumination and colour of the background on R, G, B values and accuracy of fat estimation in pork trimmings using video image analysis (VIA). Illumination proved to be the main agent which influenced on R, G, B, values and amount of white and reds spots. The greatest correlation coefficient in meat determined by Soxhlet method and calculated based on VIA when green background and halogen light were used.