The interaction of methylbromfenvinfos with model and native membranes was investigated using fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH), a probe located in the hydrophobic core of the bilayer and l,3-bis-(l-pyrene) propane, a probe distributed in the outer region of the bilayer. DPH reported a broadening of the transition profile and solidifying effects in the fluid phase of liposomes formed from dimyristoyl (DMPC), dipalmitoyl (DPPC), and distearoyl (DSPC) phosphatidylcholine in the presence of 50 μM of the insecticide. Py(3)Py detected an ordering effect of the insecticide in the fluid state of the lipids and abolished pretransition in DPPC and DSPC vesicles. Cholesterol added to DMPC decreased the influence of the insecticide. The effect of methylbromfenvinfos on the fluidity of some native membranes, namely erythrocytes, lymphocytes, brain microsomes, and sarcoplasmic reticulum, depended on the cholesterol content of these membranes.
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