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Methane is a most important greenhouse gas for planetary heating and it’s produced by methanogenic microorganisms as a metabolic byproduct and creates climate change. Methanogens are ancient organisms on earth found in anaerobic environments and methane is a key greenhouse gas concerned with methanogens. Therefore here is intense interest to writing this paper. A number of experiments have already conducted to study the methanogens in various environments such as rumen and intestinal system of animals, fresh water and marine sediments, swamps and marshes, hot springs, sludge digesters, and within anaerobic protozoa which utilize carbon dioxide in the presence of hydrogen and produce methane. The diversity of methanogens, belong to the domain Archaea and get involved in biological production of methane that catalyzes the degradation of organic compound as a part of global carbon cycle called methanogenesis. Majorly in this article we summaries the diversity of methanogens and their impact on global warming.
Methane emissions from ruminant livestock are considered to be one of the more potent forms of greenhouse gases contributing to global warming. Many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of ruminant methanogens. Therefore, basic knowledge of the diversity of these microbes in breeds of buffalo is required. Therefore, the methanogenic community in the rumen of Surti buffaloes was analyzed by PCR amplification, cloning, and sequencing of methyl coenzyme M reductase (mcrA) gene. A total of 76 clones were identified, revealing 14 different sequences (phylotypes). All 14 sequences were similar to methanogens belonging to the order Methanobacteriales. Within Methanobacteriales, 12 clones (6 OTUs) were similar to Methanosphaera stadtmanae and the remaining 8 phylotypes (64 clones) were similar to unclassified Methanobacteriales. Overall, members of the Methanobacteriales dominated the mcrA clone library in the rumen of Surti buffalo. Further studies and effective strategies can be made to inhibit the growth of Methanobacteriales to reduce methane emission from the rumen which would help in preventing global warming.
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