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The purpose of this study was to determine the effect of soil properties and Cd or Pb addition on the transformations of Pb in soils and its distribution between different fractions determined according to Tessieret al. The study was carried out in model pots filled with mineral or organic soils taken from the surface soil layer. The soils showed either acidic or neutral reactions. The mineral soil (Haplic Podzols) has the granulometric composition of light loamy sand. The soils were altered with the addition of: 0 mg kg⁻¹ of Cd or Pb (control sample); 10 mg kg⁻¹ of Cd in the form of Cd(NO₃)₂; 100 mg kg⁻¹ of Pb in the form of Pb(NO₃)₂. Speciation data indicate that in non-adjusted mineral soil, lead was associated mainly with Fe-Mn oxide and residual fractions (near 80% of total Pb), and relatively small amounts of Pb with others forms. In non-adjusted organic neutral soil, the dominant fractions of Pb were Fe-Mn oxide bound (56%) and organic (24%), while in organic acidic soil: organic (42%), exchangeable (21%) and Fe-Mn oxide bound (19%) fractions. Introducing 100 mg kg⁻¹ of Pb into the mineral soil changed the Pb distribution pattern among the particular fractions, while for organic soils it did not change the Pb distribution in comparison to that of uncontaminated soils. The addition of Pb to mineral and organic soils caused an increase in Pb bound to Fe-Mn oxides and in organic neutral soil also to the organic bound fraction. In the mineral soil, significant amounts of Pb were also present in the carbonate form. A significant amount of Pb associated with the non-residual fraction (> 65%) indicates that Pb in these soils may be easily available for plants.
The influence of two levels of artificial Cd soil contamination (2 and 20 mg Cd kg-1 of soil) on the weight of oat plants, chlorophyll content in leaves, rate of photosynthesis, stomatal conductivity and transpiration rate was researched in a pot experiments with Avena sativa L. Another objective was to detect the effect of cadmium contamination of soil on the content of cadmium in the dry mass of oat panicles, stems, upper green and bottom yellow leaves and roots. The soil contamination was applied in the forms of nitrate Cd(NO3)2, chloride CdCl2 and sulphate CdSO4 2-. High correlation was found between the measured levels of photosynthesis rate, stomatal conductivity and transpiration rates, but no correlation occurred between these levels and the cadmium content in leaves. In the variants with Cd contamination, insignificantly higher levels of photosynthesis rates were observed in the measurements than in the zero variant. A 10-fold higher Cd application dose significantly manifested itself by a higher content of Cd in all the analyzed parts of plants, including generative organs. A several-fold higher Cd level was found in the roots than in other parts of the plant, whereas the lowest Cd content was observed in panicles. However, the results obtained by measuring the cadmium content in stems and green leaves were not significant. In most treatments, a notably higher Cd content was determined in bottom yellow leaves than in upper green leaves. This indicates Cd accumulation in senescent tissues and its difficult reutilization. The highest variance was discovered in treatments with the accompanying SO4 2- anion. While estimating the effect of accompanying anions on the Cd content, significant differences were observed only under the higher level of Cd contamination. The increase in the Cd content in bottom yellow leaves after CdSO4 application was significant when compared with the treatment in which Cd(NO3)2 was applied and insignificant versus the variant with CdCl2. On the other hand, a higher and more significant content of Cd in phtosynthetically active green leaves was measured in the treatment with CdCl2 than with Cd(NO3)2.
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