Chemical analyses aimed to evaluate the contents of free phenolic acids, flavonoids and tannins at different anatomical parts of three wild strawberry cultivars: ΄Regina`, ΄Rugia` and ΄Baron von Solemacher`, originating from agrotechnical experiments, were carried out at Department of Vegetable and Medicinal Plants, University of Life Sciences in Lublin in 2008–2009. The differentiation of determined biologically active substances depending on a leaf part was observed. Mean contents of phenolic acids and flavonoids were the highest at leaf blades of ΄Rugia` amounting 1.27% and 2.66%, respectively. No significant differences in tannins contents depending on a cultivar were recorded, although leaf blades accumulated significantly higher quantities of tannins than petioles.