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The aim of the study was to find out if subtoxic doses of fenitrothion (0.1 LD₅₀, LD₅₀ =166.6 mg/kg) administered to mice exposed to transient oligaemic brain hypoxia induced by bilateral clamping of the carotid arteries (BCCA) influence memory processes, movement activity, and coordination. The BCCA was carried out under ketamine + xylazine anaesthesia. Common carotid arteries were clamped for 30 min. Twenty-four hours later; the animals were injected intraperitoneally with 0.1 LD₅₀ of fenitrothion. Controls and sham-operated animals (with carotids separated but not clamped) were injected with respective volumes of bidistilled water. All the animals were trained in passive avoidance (PA) task. The examination of memory retention in PA was done 24 h later followed by testing of fresh spatial memory in a Y- maze, movement co-ordination, and spontaneous movement activity in a 30-min period. Fenitrothion did not significantly alter memory processes in the examined mice. However, the movement coordination was significantly impaired in animals that underwent BCCA alone as well as being oligaemic and under the influence of fenitrothion vs control groups. The same groups demonstrated significantly impaired spontaneous movement activity vs controls.
The purpose of the present study was to examine whether the effects of exposure to 0.1LD₅₀ of bifenthrin on memory processes, movement activity, and coordination could be exacerbated by transient reduction of cerebral oxygen supply. The transient occlusion of both common carotid arteries (BCCA) in adult mice was performed under anaesthesia. Intraperitoneal LD₅₀ for bifethrin was estimated to be 16.1mg/kg b.w. The memory retention was evaluated in a step-through passive avoidance task (PA), working spatial memory in a Y-maze, movement coordination on a rota-rod, and movement activity in an automated device. Long-term memory impairment caused by bifenthrin was exacerbated by BCCA. Movement co-ordination was significantly altered in animals treated with the compound. Movement activity was slightly decreased in animals after BCCA and pesticide injection. These results indicate that cerebral oligaemic hypoxia potentiates long-term memory impairing effect of bifenthrin.
Preliminary study of memory processes in Mongolian gerbil (Meriones unguiculatus). Mongolian gerbil (Meriones unguiculatus) a small rodent living in the wild steppes of Mongolia was discovered in 1866 and it is becoming increasingly popular as a pet. The present work is an introduction to describe the behavior of this species and to investigate the influence of gender and age on memory process. 40 gerbils divided into four study groups (young males, young females, adult males, adult females) were twice tested in a modified version of the Lashley III maze (repeat after a week). Using statistical tests memory parameters, the activity of animals and behavior associated with the level of stress were analyzed. Conducted observations and calculations performed showed no difference between the groups (p > 0.05), which leads to the conclusion that in Mongolian gerbils age and sex have no effect on memory.
Background. Animals acquire new skills due to conditioned responses resulting from a sequence of neutral and unconditioned stimuli affecting their bodies; there is a constant time interval between the stimuli. Both stimuli become associated after a number of repetitions and animal bodies start to respond to the initially neutral stimulus as to the unconditioned one. The aim of this study was to determine whether exposure of fish to a pyrethroid—deltamethrin (an active ingredient in Decis 2.5 EC pesticide that disturbs the proper operation of the nervous system) affects the ability to learn in common carp. Materials and Methods. Production of new types of behaviour was triggered in fish by using the unconditioned stimulus (an irritating electric impulse) and the conditioned stimulus (light). The experiment was performed in a tank divided into two compartments where fish could swim freely from one compartment to another. The ability to learn was assessed on the basis of the following types of behaviour evoked in response to the conditioned stimulus: locomotor agitation, preference to occupy or avoid certain areas in the tank, an escape response, etc. Results. In fish exposed to 0.35 μg · L–1 deltamethrin (for common carp concentration 10 times lower than lethal) for 35 min the ability to produce and remember the conditioned defence response was reduced and time interval since presenting the conditioned stimulus till occurring the initial signs of the conditioned response was prolonged. Conclusion. The classical conditioning method revealed that sublethal concentration of deltamethrin restricted ability to learn and retain information in common carp, despite absence of observable intoxication symptoms.
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