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Shelf life extension of shrimp (white) using modified atmosphere packaging

100%

Kalleda R. K., Han I. Y., Toler J. E., Chen F., Kim H. J., Dawson P. L.

Polish Journal of Food and Nutrition Sciences

2013

tom 63

nr 2

Wild caught shrimp can have a shortened shelf life compared to farm raised shrimp due to handling and on-ship limitations. The loss of freshness in shrimp is partly due to autolytic reactions caused by endogenous enzymes such as polyphenol oxidase. The objective of this study was to determine the effect of sulfi tes combined with Modified atmosphere packaging (MAP) on the shelf life of non-frozen shrimp. Fresh South Atlantic white shrimp were subjected to one of four treatments, no bisulfite rinse-air packaged, 1.25% bisulfite rinse-air packaged, 1.25% bisulfite rinse-MAP (60% CO2, 18% O2, 22% N2) and 1.25% bisulfite rinse-MAP (36% CO2, 64%N2). The quality and freshness of shrimp was measured by determining total aerobic bacterial populations, package gas headspace analysis, shrimp volatiles (GC-MS), meat pH, nucleotide degradation, and visual analysis. Fresh non-frozen shrimp treated with a combination of sulfites and MAP maintained the shelf life of fresh shrimp up to 10 days while shrimp in non-MAP without sulfite and non-MAP with sulfite developed black spots within 2 and 6 days, respectively. Both MAP treatments slowed bacterial growth while the MAP with high CO2 and with O2 was more effective in preventing off odors and nucleotide degeneration.

Splenic melanosis during normal murine C57BL/6 hair cycle and after chemotherapy

80%

Michalczyk-Wetula D., Salwinski A., Popik M., Jakubowska M., Plonka P. M.

Acta Biochimica Polonica

2013

tom 60

nr 3

Cancer chemotherapy is associated with serious side effects, including temporary hair loss and impairment of pigmentation. We suspect that ectopic melanin deposition occurring due to chemotherapy may add to these effects worsening the already unpleasant symptoms. We associated the ectopic occurrence of follicular melanin after chemotherapy with splenic melanosis - an interesting example of extradermal melanin localization - and we expected an increase in splenic melanin deposition after chemotherapy. Using the C57BL/6 murine model of synchronized hair cycle induced by depilation, we visualized splenic melanin by means of several histological and histochemical protocols of staining: hematoxylin and eosin, May-Grünwald-Giemsa and Fontana-Masson. Unexpectedly, the splenic deposition of melanin decreased due to application of cyclophosphamide (i.p. 120 mg/ kg body weight on day 9 post depilation). The drop was abrupt and lasted for at least 5 days (day 13-18 post depilation), as compared with normal hair cycle. Moreover, in mice with normal, depilation-induced hair cycle we observed a similar drop shortly before entering catagen (day 15 post depilation), followed by a slow and partial increase in splenic melanization up to day 27 post depilation in both groups. We conclude that cyclophosphamide negatively affects splenic melanization and/or extradermal transfer of ectopic melanin from the dystrophic hair follicles, but the most powerful down-regulator of splenic melanosis is normal and dystrophic catagen - the phase of hair follicle involution and re-modelling.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Polish Journal of Food and Nutrition Sciences

1 Chen F.

1 Dawson P. L.

1 Han I. Y.

1 Jakubowska M.

1 Kalleda R. K.

1 Kim H. J.

1 Michalczyk-Wetula D.

1 Plonka P. M.

1 Popik M.

1 Salwinski A.

1 Toler J. E.

2 2013

Wyniki wyszukiwania

Shelf life extension of shrimp (white) using modified atmosphere packaging

Splenic melanosis during normal murine C57BL/6 hair cycle and after chemotherapy