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Detailed analyses of the chromosome meiotic behaviour and of mitotic metaphase chromosomes (2n = 32), as well as stainability studies of pollen fertility, were carried out in order to determine the cytological status of amphidiploid Trifolium repens L. (Dutch white clover). In amphidiploid (allotetraploid) Trifolium repens L., diploid-like meiotic behaviour of chromosomes was found, with no multivalent formation, and a normal karyotype with a single pair of chromosome having a secondary constriction was observed. These characteristics indicate favourable genetic and cytological stability in nature, and high pollen fertility further enhances its usefulness in breeding.
Meiosis was observed in pollen mother cells in plants of four rye Secale vavilovii Grossh. lines (nos. 52, 109, 116, 225). The behavior of 2R chromosomes with additional heterochromatin, and of other chromosomes, was observed in diakinesis, metaphase I, anaphase I and anaphase II. 2R chromosomes with additional heterochromatin formed ring and rod bivalents without disturbances. Some other chromosomes formed heterobivalents and trivalents as well as chromosome bridges and chromosome fragments, indicating structural modifications.
Twenty field-grown genotypes of diploid Italian ryegrass (Lolium multiflorum Lam., 2n = 2x = 14) were tested for their ability to induce callus and regenerate plants. Callus cultures were initiated from segments of immature inflorescences cultured on the MS medium supplemented with 4.0 mg L⁻¹ 2,4-D. The calluses were subcultured first on the maintaining medium (MS medium with 2.0 mg L⁻¹ 2,4-D) and later on the rooting medium (MS medium with 0.2 mg L⁻¹ 2,4-D). The frequency of callus induction varied depending on the source of explant and the initial genotype. A total of 473 green plantlets were regenerated, of which 420 were established in the soil. All these plants had the morphological characteristics of Italian ryegrass. Among 372 regenerants analysed cytologically, 302 (81.2%) had the expected diploid chromosome number (2n = 2x= 14), 65 (17.5%) were tetraploid (2n = 4x = 28); several aneuploids and mixoploids were also observed. All diploid and tetraploid regenerants were male and female fertile. However, a great variation of female fertility within and between both groups of regenerants was observed.
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