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Relationship between some weather factors, maize streak virus genus mastrevirus incidence and vector populations in northern Nigeria

100%

Alegbejo M. D., Banwo O. O.

Journal of Plant Protection Research

2005

tom 45

nr 2

99-105

Some aspects of the epidemiology of Maize streak virus (MSV) genus Mastrevirus concerning virus incidence, vector populations and some environmental factors were investigated in field experiments conducted over a three year period (2000–2002) at Samaru, northern Nigeria. Significant positive correlations were obtained between number of leafhoppers caught and MSV incidence and age of plant at infection and also with temperature. Also significant negative correlations were obtained between MSV incidence and mean relative humidity; between number of leafhoppers and age of plants and with mean rainfall. Leafhopper vectors caught included Cicadulina arachidis China, C. mbila Naude, C. triangula Ruppel and C. similis China, in order of abundance. Leafhopper incidence was highest in the months of September and October.

Search for variability among mastreviruses showing symptoms of maize streak virus from different regions of Nigeria by polymerase chain reactions

70%

Oluwafemi S., Thottappilly G., Alegbejo M. D.

Journal of Plant Protection Research

2008

tom 48

nr 4

529-539

Field surveys were undertaken in 1997-1999 across five ecological zones in Nigeria to collect grass species showing symptoms of Maize streak virus (MSV) (Genus Mastrevirus: Family Geminiviridae). Apart from maize (Zea mays L.), 15 grass species were found with symptoms similar to MSV. These hosts showed two types of symptoms viz: mild or severe. Polymerase chain reaction (PCR) and immunocapture IC-PCR were used to diagnose the mastreviruse. Variation among the isolates is illustrated in relationship dendograms. The PCR dendogram showed 35-85% variation among the isolates while the IC-PCR dendogram showed 55-85%. The PCR dendogram clusters the isolates essentially along symptom severity. Isolates that were most distantly related to a standard MSV strain showed mild symptoms in their grass hosts. Simple PCR protocols could help in studying diversity among grass Mastreviruses where facilities for genome sequencing are not available.

Ograniczanie wyników

2 Journal of Plant Protection Research

2 Alegbejo M. D.

1 Banwo O. O.

1 Oluwafemi S.

1 Thottappilly G.

1 2008

1 2005

Wyniki wyszukiwania

Relationship between some weather factors, maize streak virus genus mastrevirus incidence and vector populations in northern Nigeria

Search for variability among mastreviruses showing symptoms of maize streak virus from different regions of Nigeria by polymerase chain reactions