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Characterization and mass spectrometry analysis of aminopeptidase N from Pseudomonas putida Lup

100%

Jankiewicz U., Swiontek-Brzezinska M., Gorska E. B., Kowalczyk P.

Polish Journal of Microbiology

2013

tom 62

nr 4

An intracellular aminopeptidase N synthesized by Pseudomonas putida Lup was purified and characterized. The approx. 150-fold purified enzyme showed highest activity against A-β-naphthylamide at pH 7.5 and at temperature 40°C and was 100% thermostable for 240 min at 40°C. P. putida lup aminopeptidase N is a monomer with molecular mass approx. 99 kDa determined by SDS-PAGE and gel permeation chromatography. The enzyme has broad substrate specificity, but is the most active against protein substrates with N-terminal alanine and arginine. The activity of P. putida Lup aminopeptidase N is strongly inhibited in the presence of specific metallopeptidase inhibitors and is partly recovered in the presence of Zn²⁺ and Co²⁺ ions. Co²⁺, Mg²⁺ and Ca²⁺ ions increased the activity of the enzyme. Moreover, the enzyme was inhibited by inhibitors of cysteine enzymes. Analysis of fragments of the amino acid sequence of the purified enzyme demonstrated high similarity to PepN of Pseudomonas putida GB-1.

Research integrity: the experience of a Doubting Thomas

100%

Hettinger T. P.

Archivum Immunologiae et Therapiae Experimentalis

2014

tom 62

nr 2

The proteome analysis of rat platelet with nano-liquid chromatography-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique

86%

Gromotowicz-Poplawska A., Kasprzyk J., Marcinczyk N., Stepien E., Piekoszewski W., Chabielska E.

Journal of Physiology and Pharmacology

2018

tom 69

nr 6

Mass spectrometry analysis of the excretory-secretory (E-S) products of the model cestode Hymenolepis diminuta reveals their immunogenic properties and the presence of new E-S proteins in cestodes

72%

Bien J., Salamatin R., Sulima A., Savijoki K., Conn D. B., Nareaho A., Mlocicki D.

Acta Parasitologica

2016

tom 61

nr 2

Ograniczanie wyników

1 Acta Parasitologica

1 Archivum Immunologiae et Therapiae Experimentalis

1 Journal of Physiology and Pharmacology

1 Polish Journal of Microbiology

1 Bien J.

1 Chabielska E.

1 Conn D. B.

1 Gorska E. B.

1 Gromotowicz-Poplawska A.

1 Hettinger T. P.

1 Jankiewicz U.

1 Kasprzyk J.

1 Kowalczyk P.

1 Marcinczyk N.

1 Mlocicki D.

1 Nareaho A.

1 Piekoszewski W.

1 Salamatin R.

1 Savijoki K.

1 Stepien E.

1 Sulima A.

1 Swiontek-Brzezinska M.

1 2018

1 2016

1 2014

1 2013

Wyniki wyszukiwania

Characterization and mass spectrometry analysis of aminopeptidase N from Pseudomonas putida Lup

Research integrity: the experience of a Doubting Thomas

The proteome analysis of rat platelet with nano-liquid chromatography-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique

Mass spectrometry analysis of the excretory-secretory (E-S) products of the model cestode Hymenolepis diminuta reveals their immunogenic properties and the presence of new E-S proteins in cestodes