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CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16). It is shed after proteolytic degradation. Due to structural heterogeneity, CA-125 ligand capacity and biological roles are not yet understood. In this study, we assessed CA-125 as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), which is a C-type lectin showing specificity for mannosylated and fucosylated structures. It plays a role as a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor. We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays. The results showed that DC-SIGN binds to CA-125 of fetal origin and that this interaction is carbohydrate-dependent. By contrast, cancerderived CA-125 displayed negligible binding. Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens. The differences in ligand properties between CA-125 of fetal and cancer origin may be due to specificities of glycosylation. This might influence various functions of dendritic cells based on their subset diversity and maturation-related functional capacity.
Influence of application of (1,3)-(1,6)-β-D-glucans and mannans on production results of sows and piglets. The aim of the study was the assessment the production results and health status of sows and their offspring modulated by supplementation of (1,3)-(1,6)-β-D-glucans and mannans. The experiment was carried out in farrowing and nursing sector.It was performed using crossbreed (Polish Large White × Polish Landrace) pigs. The additive of (1,3)-(1,6)-β-D-glucans (G) or (1,3)-(1,6)-β-D-glucans and mannans (GM) was introduced to the feed for sows from the 85th day of gestation and for their offspring from the 22nd (after weaning) to the 90th (moving to the fattening sector) day of life. Production results were estimated basis on: changes of body weight of sows during lactation and their offspring during the experimental time, average feed intake, feed conversion ratio, mortality. To estimate health status blood samples from sows and their offspring were taken to determine the total protein level and its fraction in serum. The obtained results indicate that the additive of G or GM to lactation sows and weaned piglets can usefully influence on catabolism process in sows, growth performance and health status in piglets.
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