Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Relationship between receptors for insulin-like growth factor - I, steroid hormones and apoptosis-associated proteins in canine mammary tumors

100%

Dolka I., Motyl T., Malicka E., Sapierzynski R., Fabisiak M.

Polish Journal of Veterinary Sciences

|

2011

|

tom 14

|

nr 2

In the veterinary literature there are few data concerning the expression of insulin-like growth factor type I (IGF-IR) in the canine mammary gland tumors. The aim of the present study was the evaluation of IGF-IR expression and its correlation to the expression of estrogen receptor α (ERα) and progesterone receptor (PR), proteins: Bcl-2, Bax, p53 in canine mammary gland tumors, and also a correlation with other features: bitch’s age, tumor diameter, histologic type of tumor, degree of histologic malignancy, proliferate activity. The study was done on 112 epithelial neoplasms: 21 (19%) were adenoma, 38 (34%) complex carcinoma (adenocarcinoma), 47 (42%) simple carcinoma (adenocarcinoma) and 6 (5%) solid carcinoma. Histochemistry and immunohistochemistry methods were employed. It was shown that more common and/or higher IGF-IR expression in cells of canine mammary gland tumors was related to the histologic type of cancer of worse prognostic (solid and simple carcinoma), high histologic degree of malignancy (IIIo) but the statistical analysis did not reveal any significant differences. We observed the high degree of IGF-IR expression in tumors which displayed the high ERα and PR expression. These results suggest the involvement of IGF-IR in the development of hormonosensitive canine mammary tumors. Additionally, the significant positive correlation between expression of IGF-IR and p53, Bax was found. Our study provides some evidence that interactions exist between the IGF-IR and these apoptosis-associated proteins may contribute to the development and progression of canine mammary gland tumors. These results require further investigations.

2

Prognostic status of p53 protein accumulation in canine malignant mammary tumours

100%

Lopuszynski W., Szczubial M., Komsta R.

Bulletin of the Veterinary Institute in Puławy

|

2010

|

tom 54

|

nr 1

105-111

The expression of p53 protein was determined by the immunohistochemical study with CM-1 polyclonal antibody. The investigations were performed on formalin-fixed and paraffin-embedded tissue samples of mammary tumours obtained from 131 bitches during surgery. The p53 protein accumulation was detected in 37 tumours (28.24%). No correlation was found between p53 protein overexpression and histological type, tumour size, or regional lymph node status. However, its relationship with a histologic malignant grade approached statistical significance (P=0.067). After the 24-month follow-up period, survival analysis revealed a shortened disease-free survival and overall survival time of the dogs with tumours, which overexpressed p53 protein. Only in the case of survival time, the difference was close to the borderline of statistical significance (P=0.061). The research data presented herein, being not fully explicit, have indicated a correlation of p53 protein accumulation with worse prognosis in canine mammary tumours, although the results do not allow recognising p53 protein accumulation as a suitable prognostic factor.

3

Gene expression profiles of progestin-induced canine mammary hyperplasia and spontaneous mammary tumors

100%

Rao N. A. S., Van Wolferen M. E., Gracanin A., Bhatti S. F. M., Krol M., Holstege F. C., Mol J. A.

Journal of Physiology and Pharmacology. Supplement

|

2009

|

tom 60

|

nr 1

73-84

Spontaneous mammary tumors are the most prevalent type of neoplasms in women as well as in female dogs. Although ovarian hormones estrogen and progesterone are known to play a key role in mammary tumorigenesis, conflicting reports have been obtained from in vivo and in vitro studies concerning the role of especially progesterone in mammary tumorigenesis. Prolonged exposure to high concentrations of progesterone during the unusually long luteal phase of the estrous cycle is suspected to be the key event in canine mammary tumorigenesis. Accordingly, previous studies have shown the development of mammary hyperplasia in dogs upon prolonged progestin administration. In this study, a dog-specific cDNA microarray was used to identify oncogenic determinants in progestin-induced canine hyperplasia (CMH) and spontaneous mammary tumors (CMC) by comparing expression profiles to those obtained from mammary glands of healthy dogs. The CMH profile showed elevated expression of genes involved in cell proliferation such as PCNA, NPY, RAN and also alterations in expression of transcription factors and cell adhesion molecules. Whereas in CMC, major alterations to the expression of genes involved in cell motility, cytoskeletal organization and extra cellular matrix production was evident besides differential expression of cell proliferation inducing genes. The overall gene expression profile of CMH was related to cell proliferation where as that of CMC was associated with both cell proliferation as well as neoplastic transformation. In conclusion, our findings support a strong cell proliferation inducing potential of progestins in the canine mammary gland. Moreover, deregulated genes identified in CMC are potentially involved in their malignant and may serve as prospective therapeutic targets.

4

Circulating concentrations of insulin like growth factor-1 in female dogs with spontaneous mammary tumours

100%

Szczubial M., Dabrowski R., Lopuszynski W., Bochniarz M., Krawczyk M., Urban-Chmiel R.

Medycyna Weterynaryjna

|

2018

|

tom 74

|

nr 03

The aim of this study was the investigation of the circulating concentration of IGF-1 in female dogs with spontaneous mammary tumours. The study was performed on 34 female dogs undergoing surgery due to spontaneously occurring mammary gland tumours (24 malignant and 10 benign) and 10 clinically healthy female dogs. The serum concentrations of IGF-1 were determined by specific ELISA Kit assay. The mean concentrations of IGF-1 were significantly higher (P < 0.05) both in dogs with malignant (173.35 ± 120.45 ng/ml) and benign (130.58 ± 59.0 ng/ml) mammary tumours than in healthy controls (117.45 ± 71.0 ng/ml). In the group of female dogs with mammary carcinomas, the mean concentration of IGF-1 gradually increased from 132.85 ± 65.64 ng/ml in dogs with grade 1 tumours to 317.74 ± 119.25 ng/ml in those with grade 3 tumours, and significant differences (P < 0.05) were found among dogs with various grade tumours. These findings suggest that circulating IGF-1 may play an important role in the pathogenesis of canine mammary tumour. Moreover, high IGF-1 levels may reflect tumour cell differentiation into a more aggressive phenotype.

5

Nitroxide pirolin reduces oxidative stress generated by doxorubicin and docetaxel in blood plasma of rats bearing mammary tumor

80%

Tabaczar S., Koceva-Chyla A., Czepas J., Pieniazek A., Piasecka-Zelga J., Gwozdzinski K.

Journal of Physiology and Pharmacology

|

2012

|

tom 63

|

nr 2

6

Prognostic value of cell proliferation in canine malignant mammary tumours

80%

Lopuszynski W., Komsta R., Nozdryn-Plotnicki Z., Szczubial M.

Bulletin of the Veterinary Institute in Puławy

|

2009

|

tom 53

|

nr 2

269-276

The aim of the study was to assess the prognostic value of cell proliferation for the continuing course of the disease after the surgical excision of malignant mammary tumours in female dogs. The expression of the proliferating cell nuclear antigen (PCNA) and Ki-67 antigen has been determined by means of immunohistochemistry in formalin-fixed, paraffin-embedded tumour tissue samples taken during the mastectomy. Differences in the intensity of cell nuclei staining have been observed between the PCNA and Ki-67 antigens. The PCNA index was generally twice as high as that of Ki-67; however, the Ki-67 antigen index achieved higher values in predicting the remission time and survival time than the PCNA index. At the same time, only the mean value of the Ki-67 antigen index was statistically higher in the group of dogs with disease progression, than in the groups of dogs without recurrence and metastases. The PCNA index values demonstrated a positive correlation with the degree of histologic malignancy. The results confirm that the determination of the Ki-67 antigen index has a higher prognostic value, especially in the context of its introduction into a standard histopathological examination.

7

Immunohistochemical localisation of telomerase in mammary adenocarcinoma in bitches

80%

Nowak M., Madej J. A., Dziegiel P., Dzimira S.

Bulletin of the Veterinary Institute in Puławy

|

2008

|

tom 52

|

nr 1

The studies were aimed at immunohistochemical determination of telomerase expression in cells of primary adenocarcinomas in mammary glands of bitches. Moreover, it was aimed at comparing the obtained results with an extent of Ki-67 antigen expression, which reflects the proliferation rate of tumour cells. Material for the studies was sampled during surgery in 35 bitches of various breed, ranging in age from 5 to 14 years, which developed mammary tumour. Using histopathology, the tumours were verified as representing adenocarcinomas. This was followed by immunohistochemical reactions for the detection of telomerase and Ki-67 expression. The obtained preparations were photographed and the images were subjected to computer-assisted analysis using the MultiScaneBase V 14.02 software. The obtained mean positive correlation (r = 0.26) between the expression of Ki-67 proliferative antigen and that of telomerase in mammary adenocarcinoma of bitches points at the involvement of the enzyme in neoplastic processes in animals, in an analogy to breast cancers in women.

8

Oral beta-glucan adjuvant therapy converts nonprotective Th2 response to protective Th1 cell-mediated immune response in mammary tumor-bearing mice

80%

Baran J., Allendorf D. J., Hong F., Ross G. D.

Folia Histochemica et Cytobiologica

|

2007

|

tom 45

|

nr 2

9

Acquirement of susceptibility to vesicular stomatitis virus infection by murine resident peritoneal cells during culturing in vitro

80%

Orzechowska B., Blach-Olszewska Z.

Archivum Immunologiae et Therapiae Experimentalis

|

1996

|

tom 44

|

nr 5-6

Ograniczanie wyników

Relationship between receptors for insulin-like growth factor - I, steroid hormones and apoptosis-associated proteins in canine mammary tumors

Prognostic status of p53 protein accumulation in canine malignant mammary tumours

Gene expression profiles of progestin-induced canine mammary hyperplasia and spontaneous mammary tumors

Circulating concentrations of insulin like growth factor-1 in female dogs with spontaneous mammary tumours

Nitroxide pirolin reduces oxidative stress generated by doxorubicin and docetaxel in blood plasma of rats bearing mammary tumor

Prognostic value of cell proliferation in canine malignant mammary tumours

Immunohistochemical localisation of telomerase in mammary adenocarcinoma in bitches

Oral beta-glucan adjuvant therapy converts nonprotective Th2 response to protective Th1 cell-mediated immune response in mammary tumor-bearing mice

Acquirement of susceptibility to vesicular stomatitis virus infection by murine resident peritoneal cells during culturing in vitro