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  1. 6 Folia Histochemica et Cytobiologica

  2. 5 Acta Biochimica Polonica

  3. 2 Journal of Physiology and Pharmacology

  4. 1 Cellular and Molecular Biology Letters

  5. 1 Folia Histochemica et Cytobiologica. Supplement

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  1. 2 Dulinska J.

  2. 2 Ilendo E.

  3. 2 Laidler P.

  4. 2 Luczynski W.

  5. 2 Stasiak-Barmuta A.

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  1. 1 2015

  2. 1 2013

  3. 2 2011

  4. 2 2010

  5. 3 2009

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1

Nutritional regulation of lipogenic enzyme gene expression by SREBP-1 transcription factor in rat adipose tissue

100%
Kochan Z.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
2

The effect of dihydrotestosterone on transcription of prostatic acid phosphatase mRNA in human hyperplastic gland

100%
Dulinska J., Laidler P., Ostrowski W. S., Mrozicki S., Galka M.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 4
The effect of 5alpha-dihydrotestosterone (DHT) on the level of human prostatic acid phosphatase (hPAP) mRNA was studied using tissue slices from various benign prostatic hyperplastic glands. The absence of DHT in the incubation medium led to a gradual, significant decrease of the hPAP mRNA level. Addition of the hormone induced hPAP mRNA in a time- and dose-dependent manner. The maximal 2-4-fold induction by 10(-9) M DHT was observed after 3-5 h of incubation, and then the hPAP mRNA level was 6-20-fold higher than that in a parallel sample incubated without DHT. The results suggest that DHT is necessary to sustain the expression of hPAP in hyperplastic prostates.
3
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Insulin-like growth factor-I (IGF-1) mRNA levels and chicken muscle growth

84%
Duclos M. J.
Journal of Physiology and Pharmacology. Supplement
|
2005
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tom 56
|
nr 3
25-35
Insulin-like Growth Factor-1 is a key regulator of muscle development and metabolism in birds and other vertebrate species. In a first part, the present paper sums up the specificities of the IGF system in birds and especially those related to muscle development. In a second part, it reviews available data obtained with avian genetic or nutritional models. Data obtained by comparing genetic models with large variations of overall body growth show a positive relation between endocrine IGF-1 and growth rate. Data obtained using both genetic and nutritional models show a positive relation between muscle IGF-1 mRNA levels, which determine paracrine IGF-1 levels, and post hatch muscle growth.
4

Lack of expression of preproorexin and orexin receptors genes in human normal and prostate cancer cell lines

84%
Szyszka M., Paschke L., Tyczewska M., Rucinski M., Grabowska P., Malendowicz L. K.
Folia Histochemica et Cytobiologica
|
2015
|
tom 53
|
nr 4
5

Expression of estrogen receptors in the pelvic floor of pre- and post-menopausal women presenting pelvic organ prolapse

84%
Zbucka-Kretowska M., Marcus-Braun N., Eboue C., Abeguile G., Wolczynski S., Kottler M. L., Von Theobald P.
Folia Histochemica et Cytobiologica
|
2011
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tom 49
|
nr 3
6

The expression and localization of neuregulin-1 (Nrg1) in the gastrointestinal system of the rhesus monkey

84%
Zhao W.-J.
Folia Histochemica et Cytobiologica
|
2013
|
tom 51
|
nr 1
7

The influence of lovastatin on thrombomodulin gene expression in vascular endothelial cells - in vitro study

84%
Goralczyk K., Soszynska K., Haus O., Bielis R., Rosc D.
Folia Histochemica et Cytobiologica
|
2009
|
tom 47
|
nr 1
43-45
8

Comparative analysis of prostatic acid phosphatase and prostate-specific antigen mRNA levels in hyperplastic prostate stimulated with steroid hormones and growth factors

84%
Dulinska J., Laidler P., Labedz M.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 2
Prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA) are the mark­ers of human prostatic gland. However, it is still not completely understood if and how, steroid hormones and growth factors affect their expression and metabolism in the respect to the major pathologies of the gland. Appropriate studies were carried out on histopathologically diagnosed benign prostatic hyperplasia — BPH (n = 42) using tissue slices and cells derived from them. They were incubated with steroid hormones: 5-α-dihydrotestosterone (DHT), estradiol (E) and growth factors: epidermal growth factor (EGF), basic fibroblastic growth factor (bFGF) under culture conditions for up to 24 hours. 32P-labelled specific oligonucleotide probes were used to analyze total RNA isolated from each sample for the presence of PAP and PSA mRNAs. DHT, E, bFGF, EGF or both DHT + bFGF and DHT + EGF increased PAP and PSA mRNA levels in a time- and dose-dependent manner. The highest and statistically sig­nificant increase (P < 0.001) for PAP mRNA was observed when DHT + bFGF were present in the medium while for PSA mRNA if DHT + EGF were added to the medium. Slow but constant decrease of PAP and PSA mRNA levels was observed in the absence of each of these factors in the incubation medium. The results suggest that early expression of PSA and PAP genes and/or their mRNA stability strongly depend on DHT while differ in their response to EGF and bFGF.
9

Expression of PiT1 and PiT2 retroviral receptors and transduction efficiency of tumor cells

84%
Grabarczyk P., Wysocki P. J., Gryska K., Mackiewicz A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 2
Recombinant retroviral vectors are still the most common gene delivery vehicles for gene therapy purposes, especially for construction of genetically modified tumor vac­cines (GMTV). However, these vehicles are characterized by relatively low titre and in the case of many tumor cell lines, low transduction efficiency. We constructed bicistronic retroviral vector pseudotypes of amphotropic murine leukemia virus (A-MuLV) and gibbon ape leukemia virus (GaLV), encoding enhanced green fluorescent protein (EGFP) as a rapid and easily detectable reporter gene. Transduction of five different human melanoma and four renal carcinoma cell lines by these two virus pseudotypes revealed differences in transduction efficiency, which wase markedly lower for the renal carcinoma cell lines. Stimulation of retroviral receptor expression (PiT1 and PiT2) by phosphate depletion induced a limited in­crease of receptor mRNA levels, but did not improve the gene transfer efficiency. In contrast, simultaneous transduction with both vector pseudotypes markedly in­creased the transduction efficiency, compared to GaLV or A-MuLV alone. The same effect could be achieved by several repeated exposures of target cells to fresh vector preparation. Overexpression of GaLV receptor (PiT1) in target cells significantly increased the transduction rate and enabled retrovirus mediated gene transfer into the cells which normally are not transducible by GaLV pseudotypes. We demonstrated that, using different transduction strategies, the relatively inefficient, widely used retroviral vector systems could be significantly improved.
10
Content available remote

Effect of quercetin on paraoxonase 1 activity - studies in cultured cells, mice and humans

67%
Boesch-Saadatmandi C., Egert S., Schrader C., Coumoul X., Barouki R., Muller M. J., Wolffram S., Rimbach G.
Journal of Physiology and Pharmacology
|
2010
|
tom 61
|
nr 1
99-105
There is increasing evidence that the HDL-associated enzyme paraoxonase 1 (PON1) may have a protective function in the atherosclerotic process. An enhancement of PON1 activity by dietary factors including flavonoids is therefore of interest. Quercetin, a flavonol frequently present in fruits and vegetables has been shown to induce PON1 in cultured liver cells, but the in vivo efficacy of a dietary quercetin supplementation has yet not been evaluated. To this end, we fed laboratory mice quercetin-enriched diets with quercetin concentrations ranging from 0.05 to 2 mg/g diet for 6 weeks and determined the expression of the hepatic PON1 gene and its protein levels. Since we could establish a moderate but significant induction of PON1 mRNA levels by dietary quercetin in mice, we aimed to proof whether healthy human volunteers, given graded supplementary quercetin (50, 100 or 150 mg/day) for two weeks, would respond with likewise enhanced plasma paraoxonase activities. However, PON1 activity towards phenylacetate and paraoxon was not changed following quercetin supplementation in humans. Differences between mice and humans regarding the PON1 inducing activity of quercetin may be related to differences in quercetin metabolism. In mice, unlike in humans, a large proportion of quercetin is methylated to isorhamnetin which exhibits, according to our reporter gene data in cultured liver cells, a potent PON1 inducing activity.
11

Arginase isoenzymes in human cirrhotic liver

67%
Chrzanowska A., Gajewska B., Baranczyk-Kuzma A.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 3
465-469
 Cirrhosis leads to an inability of the liver to perform its biochemical functions. It can also lead to hepatocellular carcinoma in which, as we showed lately, arginase isoenzyme pattern changes. The present work presents our results on arginase isoenzymes and their possible role in liver cirrhosis. The study was performed on tissues obtained during liver transplantation from 60 patients with liver cirrhosis, and on samples of histologically normal liver (control) from 40 patients with benign or colorectal cancer liver metastases removed during surgery, 6-7 cm from the tumor border. Arginase isoenzymes AI (so-called liver-type arginase) and AII (called extrahepatic arginase) were identified by Western blotting and isolated by ion-exchange chromatography. Their expression on mRNA level was studied by RT-PCR. A significant decrease in arginase activity, dependent of the liver clinical stage, was observed in cirrhotic tissue. Arginase AI activity and its mRNA level were significantly decreased in cirrhotic liver, whereas the activity and expression of arginase AII were concurrently raised, as compared to normal liver. Since arginase AI is a key enzyme of the urea cycle, whereas arginase AII most probably takes part in the biosynthesis of ornithine and polyamines, the defective ammonia inactivation and increased collagen biosynthesis observed in cirrhotic liver may be related to the changes in arginase AI and AII levels, respectively.
12
Content available remote

Effect of phosphodiesterase 4 (PDE4) inhibitors on eotaxin expression in human bronchial epithelial cells

67%
Paplinska M., Chazan R., Grubek-Jaworska H.
Journal of Physiology and Pharmacology
|
2011
|
tom 62
|
nr 3
The increasing number of eosinophils into bronchoaelvolar space is observed during noninfectious inflammatory lung diseases. Eotaxins (eotaxin-1/CCL11, eotaxin-2/CCL24, eotaxin-3/CCL26) are the strongest chemotactic agents for eosinophils. Inhibitors of phosphodiesterase 4 (PDE4), the enzyme decomposing cAMP, are anti-inflammatory agents which act through cAMP elevation and inhibit numerous steps of allergic inflammation. The effect of PDE4 inhibitors on eotaxin expression is not known in details. The aim of our study was to evaluate the influence of PDE4 inhibitors: rolipram and RO-20-1724 on expression of eotaxins in bronchial epithelial cell line BEAS-2B. Cells were preincubated with PDE4 inhibitors or dexamethasone for 1 hour and then stimulated with IL-4 or IL-13 alone or in combination with TNF-. After 48 hours eotaxin protein level was measured by ELISA and mRNA level by real time PCR. Results: PDE4 inhibitors decreased CCL11 and CCL26 expression only in cultures co-stimulated with TNF-. In cultures stimulated with IL-4 and TNF- rolipram and RO-20-1724 diminished CCL11 mRNA expression by 34 and 37%, respectively, and CCL26 by 43 and 47%. In cultures stimulated with IL-13 and TNF- rolipram and RO-20-1724 decreased expression of both eotaxins by about 50%. These results were confirmed at the protein level. The effect of PDE4 inhibitors on eotaxin expression in BEAS-2B cells, in our experimental conditions, depends on TNF- contribution.
13

Influence of thrombophlebitis on TGF-beta1 and its signaling pathway in the vein wall

67%
Kowalewski R., Malkowski A., Gacko M., Sobolewski K.
Folia Histochemica et Cytobiologica
|
2010
|
tom 48
|
nr 4
14

Diminished expression of ICOS, GITR and CTLA-4 at the mRNA level in T regulatory cells of children with newly diagnosed type 1 diabetes

67%
Luczynski W., Wawrusiewicz-Kurylonek N., Stasiak-Barmuta A., Urban R., Ilendo E., Urban M., Hryszko M., Kretowski A., Gorska M.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 2
361-370
 Diabetes mellitus is one of the most common chronic diseases in children. T regulatory cells (Tregs) modulate response to autoantigens and probably play a role in pathogenesis of type 1 diabetes (T1DM). The aim of the present study was the assessment of T regulatory cells including their percentages and expression of critical genes in these cells in children with newly diagnosed type 1 diabetes. The examined group consisted of 50 children with T1DM. A flow cytometric analysis of T-cell subpopulations was performed using the following markers: anti-CD4, anti-CD25 and anti-CD127 (=IL-7R). Additionally, T regulatory cells were isolated for assessment of mRNA levels for chosen genes with the real-time RT-PCR technique. The percentages of CD4+CD25highCD127dim/- were very low and did not differ between T1DM and control children. We did not observe any statistically significant differences between healthy and diabetic children in mRNA expression for FoxP3, IL-7R (CD127), IL-8RA, IL-10RA, IL-12A, IL-2RA (CD25), IL-21, STAT1, STAT3, SOCS2, SOCS3, TGF-β1-R1, TGF-β-R2 and TBX-21 genes. Interestingly the mRNA level for CTLA-4, ICOS1, IL-23, IL-27, SMAD3 and GITR were lower in Treg cells of children with diabetes compared to the control patients. No disturbances in the percentages of T regulatory cells in patients with diabetes but diminished expression of some elements important in Treg function could be the result of an immunologic imbalance accompanying the onset of the diabetes. The results of our study should be used in future research in the field of immunotherapy in pediatric diabetes.
15

CD40L and IL-4 stimulation of acute lymphoblastic leukemia cells results in upregulation of mRNA level of FLICE - an important component of apoptosis

67%
Luczynski W., Kowalczuk O., Ilendo E., Stasiak-Barmuta A., Krawczuk-Rybak M., Malinowska I., Koltan A., Szczepanski T., Olejnik I., Jaworowski J., Chyczewski L., Matysiak M., Wysocki M., Sonta-Jakimczyk D., Wieczorek M.
Folia Histochemica et Cytobiologica
|
2007
|
tom 45
|
nr 1
16
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Infection with Echinococcus granulosus [Batsch, 1786] and expression of superoxide dismutase gene at mRNA level in hepatocytes

67%
Kozlowska-Loj J., Rzymowska J.
Wiadomości Parazytologiczne
|
2006
|
tom 52
|
nr 4
Superoxide dismutase (SOD) plays an important role in detoxication of the organism. Its function is to protect the organism against the cytotoxic action of free radicals. The highest expression of superoxide dismutase was observed in the hepatocytes adjacent to the hydatid cyst. The expression of this gene in the hepatocytes of infected livers 5 cm apart from the infection site was slightly lower.
17

Association between fertilin beta, protamines 1 and 2 and spermatid-specific linker histone H1-like protein mRNA levels, fertilization ability of human spermatozoa, and quality of preimplantation embryos

59%
Depa-Martynow M., Kempisty B., Lianeri M., Jagodzinski P. P., Jedrzejczak P.
Folia Histochemica et Cytobiologica. Supplement
|
2007
|
tom 45
|
nr 1
79-85
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