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1

The expression of GST isoenzymes and p53 in non-small cell lung cancer

100%
Oguztuzun S., Aydin M., Demirag F., Yazici U., Ozhavzali M., Kilic M., Iscan M.
Folia Histochemica et Cytobiologica
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2010
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tom 48
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nr 1
122-127
2
Content available remote

Effects of meconium on airway reactivity to histamine and acetylcholine in vitro

84%
Mokry J., Mokra D., Nosalova G.
Journal of Physiology and Pharmacology. Supplement
|
2007
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tom 58
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nr 5
3

The influence of isoprivet on neutrophil secretory action in the course of BRD in calves

84%
Wessely-Szponder J., Kloc K.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
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2010
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tom 13
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nr 4
4

Ultrastructural study of endogenous lipid pneumonia and pulmonary alveolar proteinosis coexistent with lung cancer

84%
Sulkowska M., Sulkowski S., Chyczewski L.
Folia Histochemica et Cytobiologica
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1997
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tom 35
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nr 3
5

Two-dimensional electrophoretic analysis of polypeptides expressed by normal and ovine pulmonary adenocarcinoma affected lung tissues

84%
Kycko A., Reichert M.
Bulletin of the Veterinary Institute in Puławy
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2008
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tom 52
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nr 1
Frozen lung tissue sections from 2 healthy and 2 adenocarcinoma affected sheep were lysed in appropriate buffer. The two-dimensional (2D) electrophoresis of the protein lysates was performed. The resulting gels were visualised by silver and Coomassie Blue staining, then scanned and analysed using appropriate software. There was one spot present on the image obtained from the analysis of healthy tissue and no spot was found on cancer 2D gel image. The spot was excised and analysed using mass spectrometry. As a result, cytosolic NADP-isocitrate dehydrogenase was identified. In addition, several other protein spots of different intensity in neoplastic tissues, as compared with healthy ones, were found. The last finding reflects changes in protein expression in neoplastic and healthy tissues. These preliminary results can serve as the basis for more detailed investigations of the neoplastic tissue proteome, e.g.: isoelectric focusing in narrow pH range and analysis of correlation between tissue and serum protein profiles. The analysis of serum proteins from affected sheep can reveal markers of neoplastic process and help in preclinical diagnosis of ovine pulmonary adenocarcinoma.
6
Content available remote

Oral N-acetylcysteine reverses hyperoxia-related cough suppression in guinea pigs

67%
Brozmanova M., Plevkova J., Bartos V., Plank L., Javorka M., Masar J., Tatar M.
Journal of Physiology and Pharmacology. Supplement
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2007
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tom 58
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nr 5
7

A comparison of concentrations of selected heavy metals in neoplastic and peri-neoplastic lung tissues in inhabitants from Wielkopolska and Upper Silesian Industrial District in Poland

67%
Rydzewska A.
Polish Journal of Environmental Studies
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2001
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tom 10
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nr 4
This paper presents results of a study on determination of the concentration of Cd, Pb, Cu and Zn in neoplastic and peri-neoplastic lung tissues from 110 subjects of two regions of different degrees of environmental pollution: Wielkopolska (75) and Upper Silesian Industrial District (35). The concentrations of the metals were determined by the method of differential pulse anodic stripping voltammetry (DPASV). The mean values (in mug/g wet tissue) obtained for the inhabitants from Wielkopolska were: Cd - 0.56; Pb - 0.45; Cu - 1.98; Zn - 12.93 in the neoplastic tissue and Cd - 0.88; Pb - 0.40; Cu - 1.96; Zn - 12.80 in the peri-neoplastic tissue. For the inhabitants from the Upper Silesian Industrial District (USID) the corresponding values were: Cd - 0.63; Pb - 0.25; Cu - 1.61; Zn - 20.63 in the neoplastic tissue and Cd - 1.37; Pb - 0.06; Cu - 1.66; Zn - 19.20 in the peri-neoplastic tissue. Concentrations of Cd were higher in peri-neoplastic tissues than in the neoplastic ones in the subjects from both groups. The highest concentrations of Pb and Cu were found in tissues of the inhabitants from Wielkopolska, while the highest concentrations of Cd and Zn were in tissues of those from Upper Silesian Industrial District.
8

Detection of jaagsiekte sheep retrovirus in respiratory tract fluid and lung tissue of experimentally infected lambs

67%
Kycko A., Jasik A., Reichert M.
Bulletin of the Veterinary Institute in Puławy
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2008
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tom 52
|
nr 1
The possibility of jaagsiekte sheep retrovirus (JSRV) genome detection in peripheral blood leukocytes and respiratory tract fluid of ovine pulmonary adenocarcinoma (OPA) affected sheep was tested. Five of six lambs used in the experiment were infected with JSRV by intratracheal inoculation. The blood samples were taken from all the lambs at 10-d intervals for 3 months and the white blood cells were subjected to DNA isolation followed by PCR amplification for the detection of proviral DNA that gave negative results for all the samples. The respiratory tract fluid was collected from the nostrils of lamb No. 6, which developed clinical sign of OPA 2 months after the inoculation. The fluid was examined using PCR and reverse transcriptase PCR (RT-PCR) for the presence of proviral DNA or viral RNA. The presence of OPA in the lamb was subsequently confirmed by histopathological examination and the detection of proviral DNA in the lung tissue. Results of the standard PCR amplification performed on the DNA isolated from the nasal discharge from lamb No. 6 was negative, while the RT-PCR gave positive results confirming the presence of virions in the lung fluid. Results of the study show the RT-PCR technique may be a useful tool in ante-mortem diagnosis of OPA, especially in distinguishing it from other respiratory diseases.
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