Wyniki wyszukiwania

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Gender-related effect of cold water swimming on the seasonal changes in lipid profile, ApoB/ApoA-I ratio, and homocysteine concentration in cold water swimmers

100%

Checinska-Maciejewska Z., Miller-Kasprzak E., Checinska A., Korek E., Gibas-Dorna M., Adamczak-Ratajczak A., Bogdanski P., Krauss H.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 6

2

Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects

80%

Serrano J., Cipak A., Boada J., Gonzalo H., Cacabelos D., Cassanye A., Pamplona R., Zarkovic N., Portero-Otin M.

Acta Biochimica Polonica

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2010

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tom 57

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nr 2

A previous report from our group had shown in vitro a direct interaction between peroxidases and dietary antioxidants at physiological concentrations, where in the absence of H2O2, the antioxidants could serve as oxidizing substrates for the peroxidases. However, the physiological relevance of those findings had not been evaluated. The main objective of this study was to determine whether the oxidizing products produced in the interaction between peroxidase and gallic acid at a physiological concentration of 1 μM may promote cell death or survival in a human microvascular endothelial cell line (HMEC-1). Our findings suggested that gallic acid may show a double-edged sword behaviour, since in the absence of H2O2 it may have a pro-oxidant effect which may promote cell injury (evidenced by LDH, Crystal Violet and calcein AM viability/citotoxicity assays), while in the presence of H2O2, gallic acid may act as an antioxidant inhibiting oxidative species produced in the peroxidase cycle of peroxidases. These observations were confirmed with several oxidative stress biomarkers and the evaluation of the activation of cell survival pathways like AKT and MAPK/ERK.

3

Differences between group X and group V secretory phospholipase A2 in lipolytic modification of lipoproteins

80%

Kamitani S., Yamada K., Yamamoto S., Ishimoto Y., Ono T., Saiga A., Hanasaki K.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 3

Secretory phospholipases A2 (sPLA2s) are a diverse family of low molecular mass enzymes (13–18 kDa) that hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce free fatty acids and lysophospholipids. We have previously shown that group X sPLA2 (sPLA2-X) had a strong hydrolyzing activity toward phosphatidylcholine in low-density lipoprotein (LDL) linked to the formation of lipid droplets in the cytoplasm of macrophages. Here, we show that group V sPLA2 (sPLA2-V) can also cause the lipolysis of LDL, but its action differs remarkably from that of sPLA2-X in several respects. Although sPLA2-V released almost the same amount of fatty acids from LDL, it released more linoleic acid and less arachidonic acid than sPLA2-X. In addition, the requirement of Ca2+ for the lipolysis of LDL was about 10-fold higher for sPLA2-V than sPLA2-X. In fact, the release of fatty acids from human serum was hardly detectable upon incubation with sPLA2-V in the presence of sodium citrate, which contrasted with the potent response to sPLA2-X. Moreover, sPLA2-X, but not sPLA2-V, was found to specifically interact with LDL among the serum proteins, as assessed by gel-filtration chromatography as well as sandwich enzyme-immunosorbent assay using anti-sPLA2-X and anti-apoB antibodies. Surface plasmon resonance studies have revealed that sPLA2-X can bind to LDL with high-affinity (Kd = 3.1 nM) in the presence of Ca2+. Selective interaction of sPLA2-X with LDL might be involved in the efficient hydrolysis of cell surface or intracellular phospholipids during foam cell formation.

4

Different statins produce highly divergent changes in gene expression profiles of human hepatoma cells: a pilot study

80%

Leszczynska A., Gora M., Plochocka D., Hoser G., Szkopinska A., Koblowska M., Iwanicka-Nowicka R., Kotlinski M., Rawa K., Kiliszek M., Burzynska B.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

Statins are inhibitors of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), the key enzyme of the sterol biosynthesis pathway. Statin therapy is commonly regarded as well tolerated. However, serious adverse effects have also been reported, especially during high-dose statin therapy. The aim of our study was to investigate the effect of statins on gene expression profiles in human hepatoma HepG2 cells using Affymetrix Human Genome U133 Plus 2.0 arrays. Expression of 102, 857 and 1091 genes was changed substantially in HepG2 cells treated with simvastatin, fluvastatin and atorvastatin, respectively. Pathway and gene ontology analysis showed that many of the genes with changed expression levels were involved in a broad range of metabolic processes. The presented data clearly indicate substantial differences between the tested statins.

5

Immunomodulation by statins: mechanisms and potential impact on autoimmune diseases

80%

Chow S. C.

Archivum Immunologiae et Therapiae Experimentalis

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2009

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tom 57

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nr 4

243-251

6

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The effect of NO-EDRF and monocytes-macrophages on LDL-oxidation

70%

Leitinger N., Oguogho A., Rodrigues M., Sinzinger H.

Journal of Physiology and Pharmacology

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1995

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tom 46

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nr 4

Ograniczanie wyników

Gender-related effect of cold water swimming on the seasonal changes in lipid profile, ApoB/ApoA-I ratio, and homocysteine concentration in cold water swimmers

Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects

Differences between group X and group V secretory phospholipase A2 in lipolytic modification of lipoproteins

Different statins produce highly divergent changes in gene expression profiles of human hepatoma cells: a pilot study

Immunomodulation by statins: mechanisms and potential impact on autoimmune diseases

The effect of NO-EDRF and monocytes-macrophages on LDL-oxidation