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Lipoarabinomannan as a regulator of the monocyte apoptotic response to Mycobacterium bovis BCG Danish strain 1331 infection

100%

Krzyzowska M., Schollenberger A., Pawlowski A., Hamasur B., Winnicka A., Augustynowicz-Kopec E., Niemialtowski M.

Polish Journal of Microbiology

2007

tom 56

nr 2

The mannosylated lipoarabinomannan (ManLAM) from mycobacterial species possesses strong immunomodulatory effects. Here we examined the ability of Mycobacterium tuberculosis ManLAM to interfere with the apoptotic response of mouse monocyte cell line, RAW 264.7 infected with Mycobacterium bovis BCG Danish strain. Incubation of BCG-infected monocytes with ManLAM decreased production of NO and the numbers of apoptotic cells which synergized with the polarization of mitochondrial membrane. Activities of caspase-1, -3, -8 and 9 followed pattern of apoptosis suppression by ManLAM, except for caspase-1, which showed no significant change in activity. ManLAM also stabilized anti-apoptotic ratio of bcl-2/bax expression in BCG-infected cells and blocked activation of Fas/FasL-induced pathway of apoptosis. Thus, ManLAM, apart from blocking mitochondrial pathway of apoptosis, may induce several other pathways regulating apoptotic response in BCG-infected mouse monocytes.

Gene expression profiling of lipoarabinomannan-treated mouse macrophage cultures infected with Mycobacterium bovis BCG

100%

Krzyzowska M., Malewski T., Hamasur B., Augustynowicz-Kopec E., Schollenberger A., Pawlowski A., Niemialtowski M.

Polish Journal of Microbiology

2008

tom 57

nr 2

The mannosylated lipoarabinomanan (ManLAM) from mycobacterial species possesses strong anti-apoptotic action. Here we examined the ability of ManLAM isolated from Mycobacterium tuberculosis H37Rv to alter expression profiles of apoptosis-related genes in mouse macrophages infected with Mycobacterium bovis BCG Danish strain. ManLAM suppressed BCG-induced apoptosis and activities of caspase -1, -3, -8 and 9. Mouse Apoptosis Gene Array showed that ManLAM significantly down-regulated pro-apoptotic and proinflammatory genes: caspase -1, -3, -7, -8 and -9, TNF-α/TNFSF2, Fas/TNFRSF6, Bax-α, as well as IL-12 p35 and iNOS simultaneously up-regulating anti-apoptotic genes such as Bcl-2 and Mcl-1. The effect of ManLAM was contrary to BCG-induced up-regulation of pro-apoptotic and pro-inflammatory genes and consistent with the functional data.

Lipidy sciany komorkowej rodzaju Mycobacterium

80%

Rumijowska-Galewicz A., Korycka-Machala M., Sedlaczek L.

Postępy Mikrobiologii

2002

tom 41

nr 3

235-258

Ograniczanie wyników

2 Polish Journal of Microbiology

1 Postępy Mikrobiologii

2 Augustynowicz-Kopec E.

2 Hamasur B.

2 Krzyzowska M.

2 Niemialtowski M.

2 Pawlowski A.

2 Schollenberger A.

1 Korycka-Machala M.

1 Malewski T.

1 Rumijowska-Galewicz A.

1 Sedlaczek L.

1 Winnicka A.

1 2008

1 2007

1 2002

Wyniki wyszukiwania

Lipoarabinomannan as a regulator of the monocyte apoptotic response to Mycobacterium bovis BCG Danish strain 1331 infection

Gene expression profiling of lipoarabinomannan-treated mouse macrophage cultures infected with Mycobacterium bovis BCG

Lipidy sciany komorkowej rodzaju Mycobacterium