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A reliable and sensitive liquid chromatography-electrospray ionisation tandem mass spectrometry analytical method has been developed for the determination of lincomycin in honey samples. After extraction with phosphate buffer by ultrasound, the extracted solution was subjected to the polymeric solid-phase extraction cartridge to remove endogenous compounds. The analysis was carried out on a triple-quadropule tandem mass spectrometer in the multiple reaction monitoring (MRM) mode via electrospray interface operated in positive ionisation modes. The procedure was validated in accordance with the European Commission Decision 2002/657/EC. The mean recovery of the analyte was 80%, with the corresponding intra- and inter-day variation less than 10% and 15%, respectively. Decision limits (CCα) and detection capability (CCß) were 5.60 and 6.11 µg kg⁻¹, respectively.
Twenty-four dogs were divided into three equal groups. Lincomycin was administered intramuscularly to all groups. However, group II was given S. boulardii after lincomycin induced diarrhoea occurred, and in group III, lincomycin was administered together with S. boulardii. Lincomycin caused diarrhoea in groups I (in 7.8±1.6 d) and II (in 6.9±2 d), but it did not cause diarrhoea in group III. The durations of diarrhoea were significantly different (P<0.05) between group I (6.5±1.2 d) and group II (2.9±0.4 d). The total short chain fatty acid (TSCFA) level was significantly lower (P<0.001) compared with their baselines in groups I and II at the time of the diarrhoeas' onset and cessation. Additionally, there were differences between groups at the time of diarrhoea cessation and 1 week after the treatment (P<0.05). In conclusion, lincomycin decreased TSCFAs causing diarrhoea in the dogs when given alone, and S. boulardii was effective in treating lincomycin induced diarrhoea and to prevent the occurrence of diarrhoea when given together with lincomycin.
Contagious ecthyma (CE) is a highly contagious viral skin disease that is typically self-limited. Treatment options include topical antiseptics, such as KMNO₄, local antibiotics and systemic antibiotics to prevent secondary skin infections. The aim of this study was to compare the effectiveness of the lincomycin/spectinomycin combination and dexpanthenol (Dxp) in goat kids with CE. The study was conducted at a Saanen dairy goat farm in Bursa, Turkey. The owner of the goat herd inquired at the veterinary hospital about the appearance of granulomatous lesions on the muzzles of goat kids. In this study, 24 goat kids (1-month-olds) were used. All animals were subjected to the same conditions. Blood and papule samples were taken from the animals’ lips, muzzle and buccal mucosa for virological analysis. Swab samples were taken from the lesions for culture and antibiogram. The animals were divided into three groups. Lesions were clinically scored at days 1, 7 and 15 according to a modified previously used scoring system. Goat kids were equally grouped on the basis of lesions on the buccal mucosa, lips and muzzle. The animals in group A received 15 mg/kg lincomycin/ spectinomycin combination (Lypectin®, Vilsan) intramuscularly for 3 consecutive days, group B received 20 mg/kg Dxp (Bepanthen® amp, Bayer, Germany) intramuscularly for 3 consecutive days, and group C received 0.9% NaCl (2 ml), the control treatment. Clinical recoveries were almost equal in all groups, but by day 14, group A showed better recovery than group B and group C. Both study groups also showed better results than the control group for all days. In conclusion, we believe that the lincomycin/spectinomycin combination or dexpanthenol can be useful in the supplementary treatment of CE in goat kids.
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