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The quantity of the crop plant pests have to be under control. A defense against pest organisms with toxic chemicals has been the prevailing pest control strategy for over 50 years. However, plants were found to possess insecticidal properties. Several essential oils act as antifeedants, deterrents and repellents. Essential oils are products of the secondary metabolism in plants. They are mixtures of simple and volatile compounds. One of the example is lavender oil, which repels Ceutorhynchus sp. - the most important pests of Brassica napus var. oleifera plants. The same effect was observed in the case when linalool, linalyl acetate (main components of lavender oil) and lavandulol were applied [Duda, Dubert 2008a, 2008b]. Thymol, the major constituent of thyme oil, is also a deterrent for the lepidopteran pest - Plutella xylostella L. [Akhtar, Isman 2004].
Plants under attack of herbivores can emit increased amounts of volatile compounds from their leaves. Similarly, mechanically-injured plants can emit volatile chemicals that differ both quantitatively and qualitatively from undamaged plants. In this experiment, mechanical injury increased the release of the secondary metabolites linalool (3,7-dimethyl-1,6-octadien-3-ol) and linalool oxide (5-ethenyltetrahydro-2-furanmethanol) by wheat plants. The amounts released varied significantly with injury type and the period of time after injury. The time interval for the volatile collection within the photophase also influenced the amount collected for each day. The increased emission of these compounds, as a result of injury, may be explained as a defense mechanism against wounding. The role of these plant volatiles can be further investigated in the context of plant response to mechanical injury, within the broader context of all types of injury
adano wpływ wybranych składników olejków chmielowych: linaloolu, geraniolu i α-terpineolu na właściwości sensoryczne piwa oraz podjęto próbę określenia optymalnych zawartości tych związków w piwie. W tym celu do piwa o zawartości 12% ekstraktu i 20 mg/l izozwiązków, wyprodukowanego w skali mikrotechnicznej z brzeczki przemysłowej oraz z chmielu aromatycznego ,,Lubelski", dodawano jednocześnie w różnych stężeniach linalool, geraniol i α-terpineol. Przygotowane próbki oceniano pod względem smaku, zapachu i goryczy przy udziale 8 degustatorów. Ze statystycznego opracowania punktowych ocen sensorycznych wynika, że w wyższych stężeniach linalool, geraniol i α-terpineol wywierają negatywny wpływ na cechy sensoryczne piwa, przy czym w przypadku geraniolu wpływ ten jest największy. W niskich stężeniach wpływ linaloolu i α-terpineolu jest pozytywny.
The yield and composition of essential oil obtained from the fruits of coriander (Coriandrum sativum L.) cultivated in Poland were investigated. Experiments were carried out by means of the hydrodistillation method using Clevenger and Deryng apparatus. No effect of the apparatus’ construction on the essential oil yield was observed during a 2-h process (yield of 1.62%). The composition of the coriander essential oil was determined by using gas chromatography with mass spectrometry (GC-MS). The main components were linalool (78.45%), α-pinene (5.03%), camphor (3.90%), γ-terpinene (3.80%), D-limonene (2.58%) and geranyl acetate (2.13%). Moreover, the influence of different factors, such as time of hydrodistillation, sodium chloride addition and method modification by using microwave heating on essential oil yield, was studied. The addition of sodium chloride to distilled water did not affect the yield of essential oil, but slightly increased the content of camphor. Replacing conventional heating with microwaves during 15-min experiments enhanced the yield of essential oil from 1.20 to 1.35% and had a slightly greater influence on its composition, increasing the contents of some less volatile components: linalool, camphor, geraniol, p-cymene and D-limonene. The results obtained indicate that microwave heating may be a supporting method for the production of coriander essential oil using the hydrodistillation process.
The experiment was conducted in the Research Institute of Medicinal Plants in Poznań, Poland in 2005–2006 and concerned a collection of ten sweet basil cultivars such as: ‘Minette’, ‘Mittelgrossblättriges Grünes’, Piccolino’, ‘Rubin’, ‘Red Rubin’, ‘Genoveser’, ’Martina’ and two Polish cultivars: ‘Wala’ and ‘Kasia’. Moreover, ‘Lemonette’ cultivar of Ocimum americanum L. was also included in the collection. The content of essential oil in the dried herb was estimated and its composition was analyzed. The content of essential oil in the dried herb without stems ranged from 0.23% (‘Red Rubin’) to 1.67% (‘Kasia’). The main component of the oils that came from nine basil cultivars was linalool, (from 37.07% for ‘Piccolino’ to 76.22% for ‘Minette’). Considerable amounts of 1,8-cineol and eugenol, next β-pinene, α-terpinene and myrcene were also found. The content of 16 identified components in oil represented from 69.34% (‘Piccolino’) to 96.11% (‘Wala’) of the GC profiles. Both Polish cultivars were distinguished by high content of geraniol in essential oil.
Przedstawiono najważniejsze kryteria i zasady oceny jakości chmielu stosowane przez przemysł piwowarski oraz handlowców i przetwórców, opisane w Polskiej Normie (PN-R-50255 „Chmiel Piwowarski"). Z punktu widzenia przede wszystkim przemysłu piwowarskiego najważniejszym kryterium jakościowym chmielu goryczkowego i jego produktów jest stała, niezmienna w czasie i zgodna z deklaracją producenta zawartość w nim alfa-kwasów, a dla chmielu aromatycznego i jego produktów zawartość składników olejków chmielowych odpowiedzialnych za powstawanie w piwie charakterystycznego zapachu i smaku chmielowego. Dla handlowców i przetwórców, a także dla przemysłu piwowarskiego istotna jest tzw. jednorodność odmianowa chmielu, wilgotność chmielu, jak również rodzaj stosowanych przez plantatora środków ochrony roślin.
The aim of the study was to examine the antibiofilm activity of selected essential oils (EO): Lavandula angustifolia (LEO), Melaleuca alternifolia (TTO), Melissa officinalis (MEO) and some of their major constituents: linalool, linalyl acetate, α-terpineol, terpinen-4-ol. Biofilms were formed by Staphylococcus aureus ATCC 29213 and Escherichia coli NCTC 8196 on the surface of medical biomaterials (urinary catheter, infusion tube and surgical mesh). TTC reduction assay was used for the evaluation of mature biofilm eradication from these surfaces. Moreover, time-dependent eradication of biofilms preformed in polystyrene 96-well culture microplates was examined and expressed as minimal biofilm eradication concentration (evaluated by MTT reduction assay). TTO, α-terpineol and terpinen-4-ol as well as MEO, showed stronger anti-biofilm activity than LEO and linalool or linalyl acetate. Among the biomaterials tested, surgical mesh was the surface most prone to persistent colonization since biofilms formed on it, both by S. aureus and E. coli, were difficult to destroy. The killing rate studies of S. aureus biofilm treated with TTO, LEO, MEO and some of their constituents revealed that partial (50%) destruction of 24-h-old biofilms (MBEC₅₀) was achieved by the concentration 4-8×MIC after 1 h, whereas 2-4×MIC was enough to obtain 90% reduction in biomass metabolic activity (MBEC₉₀) after just 4 h of treatment. A similar dose-dependent effect was observed for E. coli biofilm which, however, was more susceptible to the action of phytochemicals than the biofilms of S. aureus. It is noteworthy that an evident decrease in biofilm cells metabolic activity does not always lead to their total destruction and eradication.
Artemisia aucheri Boiss. is a shrub from Asteraceae family has been widespread in Iran. In traditional medicine, A. aucheri is used for its astringent, disinfectant, antimicrobial and antiparasitic properties. The aim of this study was to evaluate the chemical composition and antimicrobial activity of essential oil from aerial parts of A. aucheri against different microorganisms including Gram positive, Gram negative bacteria, filamentous fungi and yeast by disc diffusion and micro broth dilution assays. The antioxidant activity of A. aucheri essential oil was evaluated by DPPH free radical scavenging system. Fifty five components were identified by GC and GC/MS analysis and quantified from the essential oil of A. aucheri, representing 98% of total oil. The major components were geranyl acetate (17.2%), E-citral (17.1%), linalool (12.7%), geraniol (10.7%), Z-citral (10.5%). The antimicrobial results showed that Pseudomonas aeruginosa was resistant to the oil and Staphylococcus aureus and Candida albicans showed the best sensitivity to the oil. The A. aucheri has powerful antioxidant activity than that of Trolox. Some investigations were be done for evaluating the efficacy of essential oil.
The study aimed to determine agronomic characteristics and essential oil components of different basil ecotypes in semi-arid climatic conditions of South Eastern Anatolia, Diyarbakir, Turkey. Two-year harvest data about fresh and dry herb yield, dry leaf yield, essential oil content and its components from the plants of year 2015 and 2016 was analyzed in this study. Essential oil components were detected by gas chromatography/ mass spectrometry (GC/MS). The resulting outcomes demonstrated that both ecotypes and harvests had important effects on fresh and dry herb, dry leaf yield and essential oil contents of sweet basil. The highest dry leaf yield was noted from green leafy ecotypes and from second harvest stage. Twenty-three constituents were detected in the essential oil of O. basilicum ecotypes. The main components of basil essential oil were linalool, methyl chavicol, neral, geranial and methyl cinnamate that differed according to ecotypes and harvests during experimental years 2015 and 2016. Purple leafy basil ecotypes were determined as linalool rich, while greenish leaf ecotypes were abundant in methyl chavicol. Silbe – green ecotype contains higher neral and geranial levels than the other ecotypes. It was concluded that basil plant could be grown successfully and harvested two or more times to prefer for maximum dry leaf yield and essential oil contents under semi-arid climatic conditions.
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