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Influence of selected technological treatments on the BLV provirus DNA occurring in the tissues of leukaemic cattle

100%

Reichert M., Grundboeck-Jusko J., Rulka J., Stec J., Kozaczynski W.

Bulletin of the Veterinary Institute in Puławy

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1994

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tom 38

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nr 2

2

Alpha-naphthyl-acetate esterase [ANAE] and acid phosphatase [ACP] activities in lymphocytes of cows affected with bovine leukaemia virus [BLV]

100%

Grundboeck M., Szczotka M.

Bulletin of the Veterinary Institute in Puławy

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1993

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tom 37

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nr 1

3

Determination of IgG1, IgG2, and IgM antibody titres in the sera of bovine leukaemia virus - infected cows using the modified indirect ELISA

100%

Kozaczynska B.

Bulletin of the Veterinary Institute in Puławy

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1996

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tom 40

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nr 2

The aim of this study was to determine the titre of IgG₁, IgG₂, and lgM antibodies against bovine leukaemia virus (BLV) in the serum of either experimentally or naturally infected animals. Experimental infection performed in calves injected intravenously with blood from leukaemic cattle. The presence of BLV antibodies belonging to lgG₁, IgG₂, and IgM classes were studied for 7 months using the ELISA. Subsequent examinations were carried out on 126 cows from breeding stations extensively affected with BLV. Serological examinations of antibodies against BLV was performed by the ELISA. In the sera of experimentally infected animals IgM antibodies attained a maximum level in stage 2 (42 to 64 d) but lgG₁ antibodies during the last stage of immunogenesis ( 182 to 224 d). No statistically significant differences in IgG₂ antibodies throughout the entire experimental period were found. In the case of natural infection the IgG₁ and IgG₂ antibody levels increased concomitantly with the total antibody titres in the serum; however, IgG₂ values were significantly lower throughout the entire period of the study. In contrast, the highest lgM titre value was found in animals with the lowest level of total antibodies. Enzootic bovine leukaemia (EBL) is a neoplastic disease, associated with unlimited and irreparable proliferous alterations induced by bovine leukaemia virus (BL V) (25). Lymphocytes B are regarded as the target cells although T lymphocytes may also be infected (27, 30). Bovine leukosis reveals a long-lasting latency period involving integration of the viral genetic material with the host cell genome in the proviral form. A chronic lymphocytosis develops in about 30% of infected animals (1, 6, 27) whereas a small percentage of infected cattle develops tumours (6, 7). The production of antibodies against BLV is a permanent feature in the affected animals. The influence of BLV infection on immunoglobulin levels in sera has been extensively investigated but the results are contradictory (8, 9, 12, 13, 22, 29, 31). These investigations have mainly focused on total lg level measurements; consequently conflicting results concerning the behaviour of immunoglobulins regarded as the specific antibodies to the virus have been obtained.

4

Isolation of tumour associated antigen from bovine lymphoid tumours and detection of TAA - antibodies in cows affected with bovine leukaemia virus

88%

Bicka L., Grundboeck-Jusko J., Kuzmak J., Jedrzejewska M., Kozaczynska B.

Bulletin of the Veterinary Institute in Puławy

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1993

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tom 37

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nr 2

5

Influence of different physical and chemical factors on the viability of lymphocytes from leukaemic cattle and on the activity of the virus associated with those cells

75%

Grundboeck M., Buzala E., Szczotka M.

Bulletin of the Veterinary Institute in Puławy

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1993

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tom 37

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nr 1

Ograniczanie wyników

5 Bulletin of the Veterinary Institute in Puławy

2 Grundboeck M.

2 Grundboeck-Jusko J.

2 Kozaczynska B.

2 Szczotka M.

1 Bicka L.

1 Buzala E.

1 Jedrzejewska M.

1 Kozaczynski W.

1 Kuzmak J.

1 Reichert M.

1 Rulka J.

1 Stec J.

1 1996

1 1994

3 1993

Influence of selected technological treatments on the BLV provirus DNA occurring in the tissues of leukaemic cattle

Alpha-naphthyl-acetate esterase [ANAE] and acid phosphatase [ACP] activities in lymphocytes of cows affected with bovine leukaemia virus [BLV]

Determination of IgG1, IgG2, and IgM antibody titres in the sera of bovine leukaemia virus - infected cows using the modified indirect ELISA

Isolation of tumour associated antigen from bovine lymphoid tumours and detection of TAA - antibodies in cows affected with bovine leukaemia virus

Influence of different physical and chemical factors on the viability of lymphocytes from leukaemic cattle and on the activity of the virus associated with those cells