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Aktywnosc kwasnej proteazy asparaginowej szczepow Candida izolowanych z migdalkow podniebiennych

100%

Rozga A., Jaskolowska A., Kurnatowski P.

Wiadomości Parazytologiczne

2001

tom 47

nr 4

875-882

We have evalueated the activity of acidous asparagine protease in 48 strains of Candida isolated from homogenates of palatine tonsils from 116 patients after tonsilectomy. Strains most frequently isolated were C. albicans (66.7±6.8 %) and C. tropicalis (14.6±5.09 %). The range of proteolysis zone for Candida strains was 4.6 to 12.5 mm. Over 60% of the studied strains showed high and very high proteolytic activity (>6.6 mm) and the remaining strains with showed moderate activity (4.6-6.5). There were no strains with low medium activity (to 4.5 mm zone). The range of the number of rosettes E formed by lymphocytes obtained from the studied tonsils was 0-268.3. Lymphocytes isolated from 70 % of tonsils infected with Candida formed only 0-130 rosettes E; in the remaining cases the number ranged between 131 and 220. The number of lymphocytes T forming rosettes E isolated from palatine tonsils from patients not infected with fungi was 2.5 times higher. There was no correlation between the size of proteolysis zone and the number of rosettes E.

Ocena aktywnosci kwasnej proteazy asparaginowej szczepow Candida wyizolowanych z jamy ustnej u pacjentow z podwyzszonym ryzykiem zakazenia grzybami

80%

Rozga A., Kurnatowska A. J., Raczynska-Witonska G., Loga G.

Wiadomości Parazytologiczne

2001

tom 47

nr 4

883-890

We have evaluated the activity of acid aspartic protease in 195 strains of Candida isolated from the oral cavity of three groups of patients. The first group comprised patients with cancer of the larynx qualified for surgery, the second- patients with neoplastic disease (Hodgkin's disease, lymphoma, acute granulocytic leukaemia, lymphatic leukaemia, lung cancer, multiple myeloma, stomach cancer, breast cancer) who were not treated, the third group- patients with neoplastic diseases treated by chemotherapy. The strains of fungi were differentiated using API 20C and Api 20C AUX tests according to the protocol adopted at the Department of Medical Parasitology and Biology, Medical University of Łódż. The activity of acid protease was studied by Staib method in Rózga modification. Almost all strains showed high and very high proteolytic activity. The rang of proteolysis zone of Candida strains from the three groups of patients varied from 2,5 to 12,5 mm. We have found the mean proteolytic zones of strains isolated from groups Iand III differed statistically significantly (p<0,001). Similarly, statisticall sihnificant difference was seen between these parameters for groups II and III (p<0,05), while there was no difference between strains from group I and II.

Ograniczanie wyników

2 Wiadomości Parazytologiczne

2 Rozga A.

1 Jaskolowska A.

1 Kurnatowska A. J.

1 Kurnatowski P.

1 Loga G.

1 Raczynska-Witonska G.

2 2001

Wyniki wyszukiwania

Aktywnosc kwasnej proteazy asparaginowej szczepow Candida izolowanych z migdalkow podniebiennych

Ocena aktywnosci kwasnej proteazy asparaginowej szczepow Candida wyizolowanych z jamy ustnej u pacjentow z podwyzszonym ryzykiem zakazenia grzybami