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Production, characterization and iron binding affinity of hydroxamate siderophores from rhizosphere associated fluorescent Pseudomonas

100%

Deori M., Jayamohan N. S., Kumudini B. S.

Journal of Plant Protection Research

2018

tom 58

nr 1

Fluorescent Pseudomonas (FP) is a major group of plant growth promoting rhizobacteria and a well-known synthesizer of siderophores, which imparts a selective advantage on rhizosphere competence and their biocontrol traits. The present study was aimed at examining the factors affecting the production of siderophores and their potential biocontrol traits. Sixteen FP isolates were shortlisted based on their siderophore-producing ability in chrome azural S medium. The isolates were checked for variations in siderophore production under varying incubation times, temperatures, pH, iron (Fe3+) concentrations and mutagens. In addition, the iron binding affinity of siderophores, mycelial inhibition assay and plant growth promotion traits were assessed. Results showed that the siderophore production was highly influenced by the time of incubation, changes in pH, temperature and iron concentration. Chemical characterization showed that the produced siderophores were hydroxamates. Maximum siderophore production was observed at pH 7 whereas UV and EtBr exposure invariably suppressed siderophore production drastically in all isolates. All FPs from maize rhizosphere showed excellent siderophore production which could be due to the competence in strategy-II of the plant rhizosphere and significant growth inhibition on Fusarium oxysporum. Our results suggest the inclination of siderophores to iron, in terms of various criteria affecting production and the possible role of environmental mutations that affect the natural iron harvesting mechanism.

Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato

100%

Mucha J., Guzicka M., Lakomy P., Zadworny M.

Dendrobiology

2015

tom 73

We examined the interaction between the roots of Pinus sylvestris and closely related species Heterobasidion annousum s.l. (H. annosum s.s., H. parviporum, H. abietinum) that differ in host plant preference. The aim of the current study was to determine in roots the accumulation pattern of low molecular mass compounds such as catecholate and hydroxamate derivates, oxalic acid as well as iron-reduction ability of that low molecular mass compounds, that play important roles in wood degradation and they are also involved in pathogenesis. The accumulation of catechol and hydroxamate derivates increased during the early (2–6 h) and late (24–48 h) stages of interaction and similar pattern of oxalic acid accumulation were observed. The level of catecholate derivates in P. sylvestris roots that were challenged with H. parviporum or H. abietinum correlated strongly with iron reducing ability. However, when host was exposed to H. annosum s. s. hydroxamates rather than catecholates regulated iron reducing ability. The extracellular Fe3+ reducing activity was greater for H. annosum s. s. isolates than for isolates of two other species, and reduction of ferric iron may promote oxidative burst in host cell and fungal colonization. Catecholate concentration in the presence of H. annosum s.s. contributing to host cell death, confirm iron involvement in infection success.

Ograniczanie wyników

1 Dendrobiology

1 Journal of Plant Protection Research

1 Deori M.

1 Guzicka M.

1 Jayamohan N. S.

1 Kumudini B. S.

1 Lakomy P.

1 Mucha J.

1 Zadworny M.

1 2018

1 2015

Wyniki wyszukiwania

Production, characterization and iron binding affinity of hydroxamate siderophores from rhizosphere associated fluorescent Pseudomonas

Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato