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Roles of endogenous prostaglandins and cyclooxygenase isozymes in mucosal defense of inflamed rat stomach

100%

Takeeda M., Hayashi Y., Yamato M., Murakami M., Takeuchi K.

Journal of Physiology and Pharmacology

2004

tom 55

nr 1,2

Endogenous prostaglandins (PGs) are involved in adaptive gastric protection against acute injury, and cyclooxygenase (COX)-1 is responsible for the production of PGs in this phenomenon. In the present study, we examined the effect of various COX inhibitors on gastric ulcerogenic and acid secretory responses following daily exposure of the stomach to iodoacetamide (IA) and investigated the role for COX isozyme in gastric protection under subchronic mucosal irritation. Gastric mucosal irritation was induced by addition of 0.1% IA to drinking water, and the gastric mucosa was examined on the 6th day. Indomethacin (5 mg/kg) or SC-560 (selective COX-1 inhibitor, 5 mg/kg) or rofecoxib (selective COX-2 inhibitor, 5 mg/kg) was given p.o. twice 24 hr and 3 hr before the termination of IA treatment. Giving IA in drinking water for 5 days produced minimal damage in the stomach. The damage was significantly worsened by indomethacin, resulting in hemorrhagic lesions. Both SC-560 and rofecoxib also aggravated such lesions, although the effect of rofecoxib was more pronounced. Treatment with IA decreased acid secretion in pylorus-ligated stomachs, and this change was significantly reverted by indomethacin as well as SC-560 and rofecoxib. Mucosal PGE2 content was increased following IA treatment, with apparent expression of COX-2 mRNA in the stomach, and the increased PGE2 production was significantly suppressed by SC-560 and rofecoxib as well as indomethacin. These results suggest that endogenous PGs derived from both COX-1 and COX-2 are involved in the mucosal defense of the inflamed stomach, partly by decreasing acid secretion and contribute to maintaining the mucosal integrity under such conditions.

Two disulphide bridges are present in juvenile hormone binding protein from Galleria mellonella

100%

Kolodziejczyk R., Dobryszycki P., Ozyhar A., Kochman M.

Acta Biochimica Polonica

2001

tom 48

nr 4

The hemolymph juvenile hormone binding protein (JHBP) from Galleria mellonella contains two disulphide bridges/molecule and no free Cys residues. An alignment of primary structures of other Lepidopteran JHBPs indicates that Cys residues, equivalent to Cys10,17,151,195 in G. mellonella JHBP, may be involved in -S-S- bridge formation.

The vagal afferents discharge and myoelectrical activity in the gastric hyperalgesia model in rats

80%

Krolczyk G., Gil K., Zurowski D., Jung A., Thor P. J.

Journal of Physiology and Pharmacology

2008

tom 59

nr 4

707-716

A long term exposure of the gastric mucosa to inflammatory factors is suspected to alter the normal stomach motility. The consequence of it is an abnormal sensomotor response to food causing dyspeptic symptoms. Our study aimed to investigate the vagal afferents activity and the gastro-duodenal slow wave response to the mild gastric mucosa inflammation in rats. The gastric mucosal inflammation was induced by addition iodoacetamide to drinking water for 5 days. The gastro-duodenal slow wave, vagal nerve recordings and the gastric mucosa examination were performed on 6th day. The iodoacetamide irritated gastric mucosa presented the minimal inflammatory infiltration with mast cells. The vagal afferent activity was significantly increased after iodoacetamide treatment from 0.3 ± 0.1 to 1.9 ± 0.58 Hz, (p<0.05). The gastric slow wave accurate frequencies extracted from the fast Fourier transform spectra accelerated from 0.08 ± 0.01 to 0.1 ± 0.02 Hz (p<0.05). The duodenal frequencies remained unchanged (from 0.64 ± 0.02 to 0.59 ± 0.1 Hz). These results suggest that mild gastric mucosa irritation sensitizes vagal afferents and alters gastric but not duodenal pacemaker activity which may contribute to dyspeptic sensations.

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Acta Biochimica Polonica

1 Dobryszycki P.

1 Gil K.

1 Hayashi Y.

1 Jung A.

1 Kochman M.

1 Kolodziejczyk R.

1 Krolczyk G.

1 Murakami M.

1 Ozyhar A.

1 Takeeda M.

1 Takeuchi K.

1 Thor P. J.

1 Yamato M.

1 Zurowski D.

1 2008

1 2004

1 2001

Wyniki wyszukiwania

Roles of endogenous prostaglandins and cyclooxygenase isozymes in mucosal defense of inflamed rat stomach

Two disulphide bridges are present in juvenile hormone binding protein from Galleria mellonella

The vagal afferents discharge and myoelectrical activity in the gastric hyperalgesia model in rats