Wyniki wyszukiwania

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The influence of sevoflurane on the reactivity of astrocytes in the course of the experimental intracerebral haemorrhage in rat

100%

Karwacki Z., Kowianski P., Dziewiatkowski J., Domaradzka-Pytel B., Ludkiewicz B., Wojcik S., Narkiewicz O., Morys J.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 3

40 adult Wistar rats were divided into two groups depending on the applied anaesthesia. In both groups animals were generally anaesthetized with fentanyl, dehydrobenzperidol administered intraperitoneally and midazolam given intramuscularly. In the second group (SEVO) animals received sevoflurane of 2.2 vol% end-tidal concentration. Intracerebral haematoma was produced through infusion of 100 µl of autologous blood into the striatum. Each group was divided into five subgroups depending on the length of survival period: 1, 3, 7, 14, 21 days. The astrocytic population was studied by means of anti-GFAP staining. Stereological analysis was applied to estimate the numerical density of immunoreactive cells and the distribution of their types. On 7th day of observation the density of GFAP-immunoreactive astrocytes in SEVO was lower (p<0,05) than that in the control group. In the control group, the increase (p<0.05) of per cent of activated astrocytes between the 1st and 3rd survival day was noted, which remained at this level till the end of observation. In SEVO group, the increase (p<0.05) of per cent of activated astrocytes between the 3rd and 7th day and the decrease (p<0.05) between the 14th and 21st survival day were observed. During days of observation the per cent of activated astrocytes was lower (p<0.05) in the SEVO group than that in the control group. Administration of sevoflurane during anaesthesia to animals with intracerebral haemorrhage has evoked not only the delay of the activation of astrocytes but also decrease in its level.

2

Apoptosis in the course of experimental intracerebral haemorrhage in the rat

86%

Karwacki Z., Kowianski P., Dziewiatkowski J., Domaradzka-Pytel B., Ludkiewicz B., Wojcik S., Narkiewicz O., Morys J.

Folia Morphologica

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2005

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tom 64

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nr 4

248-252

Intracerebral haematoma was produced in 25 adult rats by infusion of 100 µl of autologous blood into the striatum. The animals’ brains were removed at 1, 3, 7, 14 and 21 days after production of the haematoma. The TUNEL method was used to detect DNA fragmentation and TUNEL-positive cells were qualified. TUNEL-positive cells were already found on the first day of observation and were present for three weeks after haematoma production. These results provide evidence that programmed cell death is associated with intracerebral haemorrhage.

3

The pathophysiology of intracerebral haemorrhage

86%

Karwacki Z., Kowianski P., Witkowska M., Karwacka M., Dziewiatkowski J., Morys J.

Folia Morphologica

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2006

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tom 65

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nr 4

295-300

Spontaneous intracerebral haemorrhage carries a high mortality rate and treatment of the disease raises more questions then answers. Mass effect, ischaemia and toxicity of blood components are responsible for brain tissue damage. Initially occurring disturbances of cerebral blood flow have a temporary character and do not play a key role in the pathology of intracerebral haematoma. Oedema formatting in the 24–48 hours after intracerebral bleeding is the result of multidirectional processes. The pathological mechanism that underlines it is the function of activation of systemic complement and cascade of coagulation. In the light of these findings, further clinical and experimental investigations should be focused on these factors.

4

Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

58%

Yang K. L., Lee J. T., Pang C. J., Lee T. Y., Chen S. P., Liew H. K., Chen S. Y., Chen T. Y., Lin P. Y.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 3

Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.

Ograniczanie wyników

2 Folia Morphologica

1 Cellular and Molecular Biology Letters

1 Journal of Physiology and Pharmacology

3 Dziewiatkowski J.

3 Karwacki Z.

3 Kowianski P.

3 Morys J.

2 Domaradzka-Pytel B.

2 Ludkiewicz B.

2 Narkiewicz O.

2 Wojcik S.

1 Chen S. P.

1 Chen S. Y.

1 Chen T. Y.

1 Karwacka M.

1 Lee J. T.

1 Lee T. Y.

1 Liew H. K.

1 Lin P. Y.

1 Pang C. J.

1 Witkowska M.

1 Yang K. L.

1 2012

1 2006

2 2005

The influence of sevoflurane on the reactivity of astrocytes in the course of the experimental intracerebral haemorrhage in rat

Apoptosis in the course of experimental intracerebral haemorrhage in the rat

The pathophysiology of intracerebral haemorrhage

Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection