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Peripheral mechanisms of intestinal dysmotility in the morphine tolerant and dependent rats

100%

Banach T., Zurowski D., Gil K., Weisbrodt N. W., Rosenfeld G., Thor P. J.

Journal of Physiology and Pharmacology

2006

tom 57

nr 1

Changes of intestinal motility and transit produced by tolerance to and dependence upon morphine have been partly attributed to peripheral mechanisms. We evaluated the effect of chronic peripheral morphine administration and peripheral µ-receptor blockade on vagal afferent activity (VAA) and c-Kit positive intramuscular cells of Cajal (ICCs). Ten rats were subjected to chronic subcutaneous morphine infusion for 72 h with subsequent VAA recording. Potential frequency was evaluated within recordings before and after µ receptor blockade by D-Phe -Cys -Tyr -D-Trp -Orn -Thr -Phe -Thr (CTOP) i.p. injections. Afterwards the rats were sacrificed and intramuscular c-Kit antigen expression was assessed by image analysis within removed fragments of duodenum and ascending colon. An equal group of rats served as a control for VAA and c-Kit expression. Analysis of VAA revealed similar frequencies of potentials in morphine tolerant / dependent rats before CTOP and in the controls. CTOP increased potential frequency in the morphine group which effect was visible mostly within the first 20 minutes (p=0.01). The morphine infused animals presented also higher c-Kit expression in both the duodenum (p<0.001) and the ascending colon (p<0.001) in comparison to the control group. Results of our study may indicate the involment of both the intestinal wall and the long vago-vagal reflexes in tolerance to and dependence upon opioids.

Peripheral mechanisms of intestinal dysmotility in rats with salsolinol induced experimental Parkinson's disease

84%

Banach T., Zurowski D., Gil K., Krygowska-Wajs A., Marszalek A., Thor P. J.

Journal of Physiology and Pharmacology

2006

tom 57

nr 2

Gastrointestinal dysmotility in Parkinson's disease (PD) has been attributed in part to peripheral neurotoxine action. Our purpose was the evaluation of the salsolinol effect on intramuscular interstitial cells of Cajal (ICC), duodenal myoelectrical activity (DMA) and vagal afferent activity (VAA) in rats with experimental PD. Twenty rats were divided into 2 equal groups. Experimental PD was produced in one group by 3 weeks of the intraperitoneal salsolinol injections (50 mg/kg/day), whereas the 2-nd group served as control. DMA and VAA were recorded in both groups during fasting and stepwise - gastric distension (GD) of 10 ml. Subsequently fragments of duodenum were removed and intramuscular ICC were assessed as c-Kit antigen percentage in the duodenal muscular zone. Analyses of the fasting DMA and VAA recordings didn't reveal differences between the compared groups. During GD increase of DMA dominant frequency (p=0.04) and VAA frequency (p<0.01) was observed in the controls whereas in the salsolinol group both parameters remained unchanged. Image analysis of duodenum revealed decreased c-Kit expression in the salsolinol-injected animals (p=0.05). The results of our study may suggest the direct effect of salsolinol on both ICC and neuronal pathways of gastro-duodenal reflexes.

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

2 Banach T.

2 Gil K.

2 Thor P. J.

2 Zurowski D.

1 Krygowska-Wajs A.

1 Marszalek A.

1 Rosenfeld G.

1 Weisbrodt N. W.

2 2006

Wyniki wyszukiwania

Peripheral mechanisms of intestinal dysmotility in the morphine tolerant and dependent rats

Peripheral mechanisms of intestinal dysmotility in rats with salsolinol induced experimental Parkinson's disease