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Intra- and interindividual variability of selected quantitative features of pollen grain morphology based on the example of Rosa canina L. (Rosaceae)

100%

Wronska-Pilarek D., Jagodzinski A. M.

Dendrobiology

2012

tom 67

The objective of this study was to investigate the ranges of intra- and interindividual variability on the example of R. canina. For this purpose, four flowers were collected randomly (72 flowers in total)from 18 wild shrubs of R. canina growing in one population in Poznań (Poland)and then, from each flower, 50 correctly formed pollen grains (200 pollen grains per each individual)were selected. Inter- and intraindividual pollen grain variability was characterised based on 3600 pollen grains. They were analysed for seven quantitative features, i.e. length of polar axis (P), equatorial diameter (E), thickness of the exine along the polar axis (Exp), length of ectocolpi (Le) and P/E, Exp/P, and Le/P ratios. Our study revealed highly significant differences among flowers of the particular R. canina individuals with respect to all pollen grain biometrical features. In addition, it showed that the assessment of the full range of variability in pollen grain biometric features within one individual (shrub)was more reliable if we examined several pollen grains from several flowers than for the same number of pollen grains derived from a single flower. We also found statistically significant differences among particular individuals in all pollen grain features. This proves that in order to well characterise a population of a given species from the point of view of palynology, the plant material should derive from a possibly numerous number of individuals (shrubs).

A vitamin K epoxide reductase-oxidase complex gene polymorphism [-1639G>A] and interindividual variability in the dose-effect of vitamin K antagonists

80%

Stepien E., Branicka A., Ciesla-Dul M., Undas A.

Journal of Applied Genetics

2009

tom 50

nr 4

399-403

A daily dose of vitamin K. antagonists (VKAs) may vary and its range depends on various interrelated factors. Low responsiveness to VKA (defined as a failure to achieve a target international normalized ratio [INR]) is associated with polymorphisms of the vitamin K epoxide reductase-oxidase complex gene (VKORC1). A highly prevalent promoter single-nucleotide polymorphism (VKORC1-1639 G>A, rs17878363) impairs VKORC1 expression and determines the interindividual variability of the target INR. We studied 57 patients receiving oral anticoagulation, including 50 subjects treated with acenocoumarol (mean dose: 5.7+2.3 mg/day) and 7 treated with warfarin (mean dose: 9.6±4.2 mg/day). The indications for the use of oral anticoagulant therapy were as follows: deep-vein thrombosis (N = 23); pulmonary embolism (N = 20); arterial thrombosis (N = 5); stroke (N = 4); atrial fibrillation with transient ischemic attacks (N = 2), and history of multiple thromboembolic events (N = 3). Identification of the VKORC1 genomic variation was performed using DNA sequencing methods. The prevalence of the mutated allele (VKORC1-1639A) was 41%. The VKORC1 -1639G allele carriers required a higher daily dose of acenocoumarol (5.9+1.9 mg) than the noncarriers (4.1+3.3 mg; P < 0.001). All of 5 low responded (who failed to achieve a target INR using standard dose requirements of VKAs) were homozygous for the 1639G allele. Low responders did not differ from good responders with respect to age, gender, and body mass index. Our findings suggest the potential benefits from pharmacogenetic testing, and provide evidence that the VKORCl -1639 G>A gene polymorphism may explain at least in part the low responsiveness to acenocoumarol.

Ograniczanie wyników

1 Dendrobiology

1 Journal of Applied Genetics

1 Branicka A.

1 Ciesla-Dul M.

1 Jagodzinski A. M.

1 Stepien E.

1 Undas A.

1 Wronska-Pilarek D.

1 2012

1 2009

Wyniki wyszukiwania

Intra- and interindividual variability of selected quantitative features of pollen grain morphology based on the example of Rosa canina L. (Rosaceae)

A vitamin K epoxide reductase-oxidase complex gene polymorphism [-1639G>A] and interindividual variability in the dose-effect of vitamin K antagonists