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Purification of IHF-like protein from gram-negative bacteria in one chromatographic step

100%

Krawczyk B., Kur J.

Acta Biochimica Polonica

1996

tom 43

nr 2

We describe a fast and very efficient method of purification which yields highly purified integration host factor-like proteins in one chromatografie step. IHF-like proteins from Acinetobacter junii or Proteus vulgaris are each an a£ heterodimer (subunits of 10 and 11 kDa) similar to the IHF of Escherichia coli when analyzed by polyacrylamide gel electrophoresis. The purified IHF are able to bind to the same ihf sites as IHF of E. coli. The results presented confirm that IHF is conserved during evolution in gram-negative bacteria.

Integration host factor [IHF] applied for partial digestion by restriction endonucleases in large DNA molecules [IARC procedure]

100%

Kur J.

Acta Microbiologica Polonica

1993

tom 42

nr 2

Binding of Escherichia coli integration host factor [IHF] to the origin segment of p15A plasmid

100%

Hiszczynska-Sawicka E., Kur J.

Acta Biochimica Polonica

1995

tom 42

nr 1

The integration host factor (IHF) is a sequence-specific, histone-like, multi-fun- ctional DNA-binding and -bending protein of Escherichia coli. Characterization and functional analysis of this protein has been carried out mainly in bacteriophage λ and other mobile genetic elements. In this paper we report data concerning the binding of IHF protein to the plasmid oripl5A region. IHF binds to the single site of the DNA fragment containing the oripl5A, as shown by the gel mobility shift assays and footprinting experiment. On the basis of the ihf consensus sequences published, we have been able to identify one sequence of putative ihf site into the oripl5A sequence with two mismatches in relation to the consensus sequence of Kur et al., 1989, Gene 81,1-15. One ihf binding site was also found in the oriColEl region sequence with three mismatches in relation to this consensus sequence.

Use of IHF - mediated Achilles' heel cleavage [IHF-AC] method for mapping ihf sites

80%

Kur J.

Acta Microbiologica Polonica

1993

tom 42

nr 2

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Acta Microbiologica Polonica

4 Kur J.

1 Hiszczynska-Sawicka E.

1 Krawczyk B.

1 1996

1 1995

2 1993

Wyniki wyszukiwania

Purification of IHF-like protein from gram-negative bacteria in one chromatographic step

Integration host factor [IHF] applied for partial digestion by restriction endonucleases in large DNA molecules [IARC procedure]

Binding of Escherichia coli integration host factor [IHF] to the origin segment of p15A plasmid

Use of IHF - mediated Achilles' heel cleavage [IHF-AC] method for mapping ihf sites