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The aim of the study was to evaluate the effect of different concentrations of spermatozoa in insemination dose (ID) on fertility rate of inseminated does, and on the number of all and live-born young per litter in the breed of crossbred rabbits by using certain rates of dilution. The crossbred rabbits of female strain HYPLUS PS 19 and male strain HYPLUS PS 39 were used in the experiment. In the first part of the experiment does were inseminated with ID 0.5 cm3 with the concentrations of spermatozoa from 115 to 550 x 106 in 1 cm3. In the second part of the experiment does were inseminated with the same volume of ID (0.5 cm3), however with the lower concentration, namely from 55 to 150 x 106 in 1 cm3. The particular concentrations of spermatozoa in ID had no statistically significant effect on fertility rate and number of all and live born young per litter in both parts of experiment.
The advantages of the insemination through the cervix into the uterus using a lower number of spermatozoa were investigated. Two hundred Danube White breed sows housed on a commercial pig farm were used in the study. The sows were randomly assigned to one of the following two equal treatment groups: group 1 - post-cervical insemination (PCI) with 1.5 x 10⁹ spermatozoa in 50 ml with a SOFT QUICK® catheter, and group 2 - cervical insemination (CI) with 3.0 x 10⁹ spermatozoa in 100 ml with a spiral type catheter. The animals were inseminated twice after the onset of standing reflex. Pregnancy was diagnosed at 28 to 30 d after the insemination by ultrasonography. Prior to data analysis, the sows were categorised into two weaning-to-oestrus intervals (WEI) of ≤6 d or >6 d. The highest farrowing rate - 100% was obtained after cervical insemination with 3.0 x 10⁹ spermatozoa in sows cycling within 6 d after weaning. In both methods, there was a tendency for low farrowing in sows in the >6 d WEI category in comparison with the animals at ≤6 d WEI. Similarly, the mean litter size with the standard device (CI) were 10.35 and 11.67 and with the PCI device 10.23 and 10.35. The average number of piglets born alive per litter was greater (P<0.5) for sows inseminated by the CI method of at >6 d category. The results show that SOFT QUICK® could be used in sows in a successful way as an intrauterine method in commercial farms. The main effect of the number of spermatozoa per dose and the weaning-to-oestrus interval did not significantly influence the overall birth weight of the litter and the average birth weight of the piglets.
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