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The effect of Aea-TMOF on egg maturation in Heliothis virescens: a preliminary study

100%

Pszczolkowski M. A., Rhine C., Ramaswamy S. B., Borovsky D.

Pestycydy

2007

nr 3-4

33-38

Trypsin Modulating Oostatic Factor from the mosquito, Aedes aegypti, (Aea-TMOF) inhibits juvenile hormone (JH) - stimulated egg chorionation and patency in the follicular epithelium cells of Heliothis virescens. Aea-TMOF exhibits highest inhibitory effect on oocytes or follicular epithelium cells when it is administered together with JH I rather than with JH III. These results indicate that Aea-TMOF specifically inhibits JH I-dependent events during egg maturation in Heliothis virescens. Preliminary pharmacological analysis of the Aea-TMOF effect on patency suggests that the decapeptide hormone acts upstream of the protein kinase-dependent step during the JH activated cellular signaling pathway.

Functions of biological clocks in insect physiology and reproduction: Practical aspects

80%

Giebultowicz J. M., Bebas P.

Pestycydy

2005

nr 4

77-90

Carbonic anhydrase activity in the vas deferens of the cotton leafworm - Spodoptera littoralis [Lepidoptera: Noctuidae] controlled by circadian clock

80%

Kotwica J., Ciuk M. A., Joachimiak E., Rowinski S., Cymborowski B., Bebas P.

Journal of Physiology and Pharmacology. Supplement

2006

tom 57

nr 8

107-123

The male reproductive tract of Lepidoptera is an ideal model for the study of the physiological role of peripheral clocks in insects. The latter are significant in the generation and coordination of rhythmic phenomena which facilitate the initial stages of sperm capacitation. This process requires the maintenance of pH in the upper vas deferens (UVD) aided by, among others, H+-ATPase. Our aim was to determine the potential involvement of carbonic anhydrase (CA) in this process, an enzyme tasked with generating protons subsequently utilized by H+-ATPase to acidify the UVD milieu in S. littoralis, during the time when the lumen of this organ is filled with sperm. We attempted to answer the question whether CA activity can be controlled by the biological oscillator present in the male reproductive tract of the cotton leafworm. Using PAGE zymography, the presence of CA was demonstrated in the UVD wall, but not in the luminal fluid nor in the sperm. Using histochemistry, it was shown that CA is active in the UVD epithelium, and that this activity varies throughout the day and is most likely controlled by an endogenous biological clock. Conversely, the application of CA inhibitors, acetazolamide and sodium thiocyanate, in conjunction with an analysis of H+-ATPase activity in the acidification the UVD environment shows that CA most likely does not play a direct role in the regulation of the pH in this organ.

Ograniczanie wyników

2 Pestycydy

1 Journal of Physiology and Pharmacology. Supplement

2 Bebas P.

1 Borovsky D.

1 Ciuk M. A.

1 Cymborowski B.

1 Giebultowicz J. M.

1 Joachimiak E.

1 Kotwica J.

1 Pszczolkowski M. A.

1 Ramaswamy S. B.

1 Rhine C.

1 Rowinski S.

1 2007

1 2006

1 2005

Wyniki wyszukiwania

The effect of Aea-TMOF on egg maturation in Heliothis virescens: a preliminary study

Functions of biological clocks in insect physiology and reproduction: Practical aspects

Carbonic anhydrase activity in the vas deferens of the cotton leafworm - Spodoptera littoralis [Lepidoptera: Noctuidae] controlled by circadian clock