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Owing to its biological properties the microelement selenium has attracted enormous interest. It has been established that selenium stimulates the human immune system and has anti-carcinogenic effect. The main sources of selenium are high-protein foodstuffs of plant and animal origin, as well as high-protein dairy products. The aim of this study was to detect selenium content in confectionery products using speciation analysis in order to determine inorganic forms of selenium such as Se2- SeO32- and SeO42- anions. The hydride generation method combined with the atomic absorption spectroscopy was used for the final determination of selenium forms. The determination of selenium was conducted using aqueous extraction and digestion of samples with concentrated acids. The speciation determination of selenium was conducted in ten confectionery products. The correlation between the total content of selenium and its individual forms (-II), (IV),(VI) of different oxidation degree was also examined. It was shown that there was no correlation between the total selenium and inorganic forms of selenium. That means that speciation analysis is the only correct analysis of selenium content in foods.
The experiment was conducted to determine the effect of adequate zinc level supplementation (inorganic versus organic form) on the innate immune response of kids. The count of white blood cells, leukocyte differential count, phagocytic activity, and phagocytic index (as markers of the immune functions) were determined. Phagocyticic activity was not significantly higher in the inorganic-zinc-treated group in comparison to the control (64.7±8.91 vs 61.2 ± 9.15 %). The production of reactive oxygen species (ROS) by goat's blood neutrophils was detected by luminol-enhanced chemiluminescence (CL). CL was performed to determine integral CL, peak CL, and peak-time after addition of calcium ionophore A23187 (Cal-I), opsonised zymosan (OZP), and phorbol- 12-myristate-13-acetate (PMA). A significant ROS increase reflected in peak CL (P≤0.05) was found in the lactate-Zn-treated group when Ca-I was used as activator. In the same group there was a significant integral CL increase (P≤0.05) when we used Ca-I as activator. Others parameters showed no significant changes.
Opracowano metodę specjacyjnego oznaczania nieorganicznych form selenu (-II), (IV), (VI) oraz całkowitej zawartości selenu w próbkach produktów spożywczych uwzględniając ich złożoną matrycę. Wykorzystano do oznaczeń końcowych technikę generowania wodorków w połączeniu z metodą atomowej spektroskopii absorpcyjnej HG-AAS. Oznaczenie selenu opiera się na połączeniu ekstrakcji wodnej próbki z roztwarzaniem próbki stężonymi kwasami. Oznaczenie specjacyjne selenu wykonano w pięciu grupach produktów spożywczych: kaszach, ziołach, sokach owocowych, odżywkach dla niemowląt oraz w wybranych pokarmach stanowiących potencjalne naturalne źródła selenu.
Standard DJ feed mixture for laying hens (ISA Brown) was supplemented with organic or inorganic forms of selenium and zinc. The organic forms consisted Saccharomyces cerevisiae yeast enriched with Se (Y-Se) and Zn (Y-Zn). The inorganic forms were sodium selenite (Na-Se) and zinc oxide (ZnO). The concentrations of elements in the experimental feed were (mg·kg-1): selenium 1.414 (Y-Se) and 1.393 (Na-Se); zinc 79.3 (Y-Zn) and 78.9 (ZnO). After 6 days of feeding treatment, for the next 5 days there were collected droppings and eggs to determine Se and Zn concentrations with the use of ICP method. It was stated that in hens availability of elements (as apparent absorption) was respectively (%): Y-Se 63.65, Y-Zn 38.5, Na-Se 61.12 and ZnO 35.41. In eggs content of Y-Se hens the increase of Se was proved if compared to Na-Se eggs (p<0.05).
Many selenoorganic compounds play an important role in biochemical processes and act as antioxidants, enzyme inhibitors or drugs. The effects of a new selenocompound — bis(2-aminophenyl)-diselenide on oxidative/nitrative changes in human plasma proteins induced by peroxynitrite (ONOO−) were studied in vitro and compared with the those of ebselen, a well-known antioxidant. We also studied the role of the tested selenocompounds in peroxynitrite-induced plasma lipid peroxidation. Exposure of the plasma to peroxynitrite (0.1 mM) resulted in an increase in the level of carbonyl groups and nitrotyrosine residues in plasma proteins (estimated using the ELISA method and Western blot analysis). In the presence of different concentrations (0.025–0.1 mM) of the tested selenocompounds, 0.1 mM peroxynitrite caused a distinct decrease in the level of carbonyl group formation and tyrosine nitration in plasma proteins. Moreover, these selenocompounds also inhibited plasma lipid peroxidation induced by ONOO−1 (0.1 mM). The obtained results indicate that in vitro bis(2-aminophenyl)-diselenide and ebselen have very similar protective effects against peroxynitrite-induced oxidative/nitrative damage to human plasma proteins and lipids.
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