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1

Detection of CMV infected cells by flow cytometry - evaluation of MAbs CCH2 and AAC10 directed against early and late CMV antigens

100%
Siennicka J.
Polish Journal of Microbiology
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2004
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tom 53
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nr 2
In this work evaluation of usefulness of monoclonal antibodies (MAbs) CCH2 and AAC10 directed against early - pUL44(DB52) and late - ppUL83(pp65) CMV antigens, utilized in Department of Virology, NIH for routine diagnosis of CMV infection by shell vial and pp65 antigenemia assay, for determination of CMV antigens by flow cytometry in human leucocytes, isolated, infected and cultivated in vitro was presented.
2

Differences in inhibition of apoptosis depending on the virulence of used herpes simplex virus type 1 strains. Function of interferon alpha in apoptotic death of virus infected cells

86%
Rechnio M., Litwinska B.
Acta Microbiologica Polonica
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2003
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tom 52
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nr 3
3

Protein A-gold immunoelectron microscope analysis of the antigen activity of the structural components of bovine herpesvirus-4 [BHV-4] particles and surface antigen expression of cells infected with BHV-4

72%
Simeonov K., Polianova M., Jordanova P., Roussev R.
Bulletin of the Veterinary Institute in Puławy
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2007
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tom 51
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nr 2
Protein A-gold immuno-electron microscope (prA-g- IEM) study was performed to detect virus-induced cell surface antigens on bovine herpesvirus-4 (BHV-4) infected MDBK cells, using pre-embedding prA-g-IEM technique and polyclonal rabbit BHV-4 antiserum. The capability of serum antibodies to recognise epitopes of either capsid or virus envelope structural antigens, was preliminary determined with purified virus preparations using the same technique. Under EM observation, virtually all virions presented as nucleocapsids were intensively labelled, whilst only single gold particles were attached to the enveloped virions. This suggests that BHV-4 envelope do not appear as immunodominant viral structure. Surface immuno-labelling did not localise reactive BHV-4 antigens on membranes of infected MDBK cells, regardless of the stage of infection and mode of processing before immune reaction (fixed and unfixed cells). Those findings substantiate the low immunogenic potential of BHV-4, which could be one of the reasons for its prolonged persistence in infected animals.
4

Inhibition of proteolytic processing of adenoviral proteins by epsilon-aminocaproic acid and ambenum in adenovirus-infected cells

72%
Nosach L., Dyachenko N., Zhovnovataya V., Lozinskiy M., Lozitsky V.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 4
Maturation of adenovirus particles is markedly affected by proteolytic processing. The possibility for blocking the conversion of precursor structural core protein (preVII) into mature structure protein VII by officinal drugs £-aminocaproic acid and ambenum has been demonstrated in Hep-2 cells infected with adenovirus. Proteolytic processing may be regarded as one of the targets for inhibiting adenovirus reproduc­tion.
5
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A comparative cytological and morphometric analysis of vacuolation in central tissue of the effective and ineffective pea [Pisum sativum L.] root nodules

58%
Borucki W.
Acta Societatis Botanicorum Poloniae
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2007
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tom 76
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nr 2
Vacuoles play very important physiological roles in plant cells. Pea root nodules, which exhibit distinct zonation (meristematic zone and central tissue zones), may serve as a good experimental model for the investigations of vacuole development and its importance to cell and tissue functioning. Moreover, the nodule central tissue is composed of both infected and uninfected cells which play different physiological roles and differ in the level of vacuolation. Cytological observations revealed that central vacuoles of the infected cells of the effective nodules expand toward cell walls. Thus only thin layers of the cytoplasm separate each central vacuole from plasma membrane and cell wall. This finding is discussed from the viewpoint of improved exchange of solutes and water between the central vacuole and apoplast of the infected cell. Three-dimensional reconstruction of the vacuoles of infected cells within a fragment of effective nodule central tissue, showed their spatial arrangement. Possible advantages coming from the spatial arrangement of vacuoles within the central tissue are discussed. A comparative study of the central tissue (bacteroidal tissue) and meristem vacuolation of the effective and ineffective pea root nodules is also presented. Morphometric measurements revealed that the effective nodule central tissue was more vacuolated than the ineffective one. It was proved that maturation of the infected cells involves dynamic changes in their vacuolation. Having numerous fixing nitrogen bacteroids, the infected cells of effective central tissue were less vacuolated than uninfected cells. On the other hand, both infected and uninfected cells of the effective central tissue showed a much higher level of vacuolation in nitrogen-fixing zone than cells of the same type in ineffective tissue. These results indicate that vacuolation is an important factor in development and functioning of pea root nodule central tissue.
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