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Indirect organogenesis of faba bean (Vicia faba L. minor)

100%

Skrzypek E., Czyczylo-Mysza I., Marcinska I.

Acta Biologica Cracoviensia. Series Botanica

2012

tom 54

nr 2

Using four Polish Vicia faba L. minor cultivars (Bronto, Dino, Tibo, Nadwiślański) we obtained callus from epicotyl fragments collected from 7- and 14-day-old seedlings and from cotyledonary nodes of immature seeds. Callus induction efficiency varied from 81% to 97% depending on the origin of the explant. Shoots regenerated only from the cotyledonary nodes of all tested cultivars. On average, 50% of the explants grown on MS medium containing 1.0 mg dm-3 NAA, 0.5 mg dm-3 BAP, 0.25 mg dm-3 GA3, 1.0 g dm-3 casein hydrolysate, 750 mg dm-3 inositol, 3% sucrose and 0.4% agar were able to regenerate shoots. The number of calluses regenerating shoots was highest from explants collected from fruiting nodes 6 to 9. Decapitation of donor plants increased the percentage of calluses regenerating shoots. On half-strength MS medium with 2 mg dm-3 NAA and on 1/2 MS alone, 11% of the shoots rooted; on 1/2 MS with 1 g dm-3 AC, 8.0% rooted. The regenerants were transferred to Perlite with Hoagland medium and acclimated. Ten percent of the regenerated plants survived the acclimation process, flowered and produced seeds.

Morphology, secoiridoid content and RAPD analysis of plants regenerated from callus of Centaurium erythraea Rafn.

84%

Piatczak E., Krolicka A., Wysokinska H.

Acta Biologica Cracoviensia. Series Botanica

2011

tom 53

nr 2

Centaurium erythraea plants obtained by indirect organogenesis are described in the paper. The plants were initiated from a single adventitious shoot regenerated from callus derived from the cotyledon of a 30-day-old seedling. The shoot was multiplied on MS medium supplemented with IAA (0.1 mg·L-1) and BAP (1.0 mg·L-1). The multiplication rate (28 shoots per culture within 4 weeks) was highest at the first subculture and decreased in further subcultures. The shoots were rooted on MS medium. The effect of IBA (0.1 mg·L-1) on the number of shoots forming roots differed depending on the composition of the basal medium (MS). The rooted shoots were transplanted to soil and grown in a greenhouse with 90% effectiveness. RAPD analysis was done with adventitious shoots of C. erythraea from in vitro culture. In shoots and whole plants regenerated from the callus tissue, secoiridoid content was determined by the HPLC method. We showed significant differences in morphology (leaf size, fresh and dry weight and height of plants) and changes in the DNA profiles as compared to earlier reports for shoot tip-derived shoots and plants of C. erythraea, but the two groups of plants biosynthesized the same qualitative pattern and similar levels of secoiridoids, up to 150 mg·g-1 dry weight; the increased biomass of plants regenerated from callus tissue makes them a better source of secondary metabolites.

Phenylethanoid glycosides in Plantago media L. organs obtained in in vitro cultures

84%

Budzianowska A., Kikowska M., Malkiewicz M., Karolak I., Budzianowski J.

Acta Biologica Cracoviensia. Series Botanica

2019

tom 61

nr 1

Ograniczanie wyników

3 Acta Biologica Cracoviensia. Series Botanica

1 Budzianowska A.

1 Budzianowski J.

1 Czyczylo-Mysza I.

1 Karolak I.

1 Kikowska M.

1 Krolicka A.

1 Malkiewicz M.

1 Marcinska I.

1 Piatczak E.

1 Skrzypek E.

1 Wysokinska H.

1 2019

1 2012

1 2011

Wyniki wyszukiwania

Indirect organogenesis of faba bean (Vicia faba L. minor)

Morphology, secoiridoid content and RAPD analysis of plants regenerated from callus of Centaurium erythraea Rafn.

Phenylethanoid glycosides in Plantago media L. organs obtained in in vitro cultures